Background/Aim: To characterize global microRNA (miRNA) expression profile in the first trimester maternal plasma of women who subsequently develop late-onset preeclampsia (LOPE) compared to uncomplicated pregnancies. Materials and Methods: Five first trimester plasma samples from women who developed LOPE and 5 controls were analyzed using next generation sequencing technology (NGS) followed by target prediction, Gene Ontology analysis and pathway identification. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for confirmation in an independent cohort of 12 LOPE cases and 12 controls. Results: miR-23b-5p and miR-99b-5p were down-regulated by >1.5 fold in LOPE complicated pregnancies (p value <0.05) compared to controls. Target prediction showed that the major targets of these miRNAs are associated with glycometabolism and immune response. Conclusion: miR-23b-5p and miR-99b-5p are possibly implicated in the pathogenic mechanisms leading to the induction of LOPE and may serve as candidate non-invasive biomarkers for early prediction and prevention.

中文翻译：

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深度测序发现迟发性先兆子痫中的循环微RNA失调

背景/目的：与无并发症的妊娠相比，表征随后发展为迟发性先兆子痫 (LOPE) 的妇女在孕早期母体血浆中的整体 microRNA (miRNA) 表达谱。材料和方法：使用下一代测序技术 (NGS) 分析了 5 个患有 LOPE 的女性和 5 个对照的妊娠早期血浆样本，然后进行了目标预测、基因本体分析和通路鉴定。在 12 名 LOPE 病例和 12 名对照的独立队列中进行了定量实时聚合酶链反应 (qRT-PCR) 以进行确认。结果：与对照相比，在 LOPE 复杂妊娠中，miR-23b-5p 和 miR-99b-5p 下调 > 1.5 倍（p 值 <0.05）。靶点预测表明，这些miRNAs的主要靶点与糖代谢和免疫反应有关。结论：miR-23b-5p 和 miR-99b-5p 可能与导致 LOPE 的发病机制有关，可作为早期预测和预防的候选非侵入性生物标志物。