Background/Aim: Geranylgeraniol (GGOH), a C20 isoprenoid naturally occurs in several foods. We previously reported that GGOH treatment reduced the expression levels of Atrogin-1 which is involved in skeletal muscle degradation and stimulates the myogenic differentiation of C2C12 myoblasts. However, the effect of GGOH supplementation on skeletal muscle metabolism in vivo is unknown. Materials and Methods: Skeletal muscle atrophy was induced by denervation. The expression levels of Atrogin-1 were assessed by western blotting or real time PCR. Results: Intraoral administration of GGOH reduced the decrease in the cross-sectional area of muscle fibers and also suppressed the expression levels of Atrogin-1 in denervation induced muscle atrophy. Also, GGOH treatment suppressed the expression of Atrogin-1 and the decrease in skeletal muscle fiber size by glucocorticoid in vitro. Conclusion: Intraoral administration of GGOH rescues denervation-induced muscle atrophy via suppression of Atrogin-1.

中文翻译：

---

口服香叶基香叶醇通过抑制 Atrogin-1 来挽救去神经支配引起的肌肉萎缩

背景/目的：香叶基香叶醇 (GGOH)，一种 C20 类异戊二烯，天然存在于多种食物中。我们之前报道过 GGOH 处理降低了 Atrogin-1 的表达水平，Atrogin-1 参与骨骼肌降解并刺激 C2C12 成肌细胞的肌源性分化。然而，补充 GGOH 对体内骨骼肌代谢的影响尚不清楚。材料和方法：骨骼肌萎缩是由去神经支配引起的。通过蛋白质印迹或实时 PCR 评估 Atrogin-1 的表达水平。结果：GGOH的口内给药减少了肌纤维横截面积的减少，并且还抑制了去神经支配诱导的肌肉萎缩中Atrogin-1的表达水平。还，GGOH 治疗抑制了 Atrogin-1 的表达和糖皮质激素在体外的骨骼肌纤维大小的减少。结论：GGOH 的口内给药通过抑制 Atrogin-1 来挽救去神经支配诱导的肌肉萎缩。