Background/Aim: Oral cancer may become advanced because of delay in diagnosis. In order to promote oral cancer screening, simple and highly reliable screening methods that can be implemented at general dental clinics are required. Herein we investigated differential salivary gene expression between oral squamous cell carcinoma (OSCC) patients and healthy volunteers (HV) to identify new biomarkers for OSCC detection. Materials and Methods: Candidate genes were selected by microarrays, nuclear undecaprenyl pyrophosphate synthase 1 (NUS1) and reticulocalbin 1 (RCN1) were selected for further investigation. We used real-time quantitative reverse transcription PCR (qRT-PCR) to determine NUS1 and RCN1 expression levels in saliva and tissues. Results: qRT-PCR analysis of clinical samples revealed that OSCC patients had significantly higher expression of salivary NUS1 and RCN1 than HV. Conclusion: A combination of NUS1 and RCN1 accurately distinguished patients from controls, and this combination can be implemented as a screening test for OSCC.

中文翻译：

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唾液 NUS1 和 RCN1 水平作为口腔鳞状细胞癌诊断的生物标志物

背景/目的：口腔癌可能因诊断延迟而进展。为了促进口腔癌筛查，需要可以在普通牙科诊所实施的简单且高度可靠的筛查方法。在这里，我们研究了口腔鳞状细胞癌 (OSCC) 患者和健康志愿者 (HV) 之间的差异唾液基因表达，以确定用于 OSCC 检测的新生物标志物。材料和方法：通过微阵列选择候选基因，选择核十一烯焦磷酸合酶1（NUS1）和网状蛋白1（RCN1）进行进一步研究。我们使用实时定量逆转录 PCR (qRT-PCR) 来确定唾液和组织中的 NUS1 和 RCN1 表达水平。结果：临床样本的 qRT-PCR 分析显示，OSCC 患者唾液 NUS1 和 RCN1 的表达明显高于 HV。结论：NUS1和RCN1的组合能准确地区分患者和对照组，这种组合可以作为OSCC的筛查试验。