Aroma, berry firmness and berry shape are three main quality traits in table grape production, and also the important target traits in grapevine breeding. However, the information about their genetic mechanisms is limited, which results in low accuracy and efficiency of quality breeding in grapevine. Mapping and isolation of quantitative trait locus (QTLs) based on the construction of genetic linkage map is a powerful approach to decipher the genetic determinants of complex quantitative traits. In the present work, a final integrated map consisting of 3411 SLAF markers on 19 linkage groups (LGs) with an average distance of 0.98 cM between adjacent markers was generated using the specific length amplified fragment sequencing (SLAF-seq) technique. A total of 9 significant stable QTLs for Muscat flavor, berry firmness and berry shape were identified on two linkage groups among the hybrids analyzed over three consecutive years from 2016 to 2018. Notably, new stable QTLs for berry firmness and berry shape were found on LG 8 respectively for the first time. Based on biological function and expression profiles of candidate genes in the major QTL regions, 3 genes (VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350) related to berry firmness and 3 genes (VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200) linked to berry shape were highlighted. Overexpression of VIT_08s0032g01110 in transgenic Arabidopsis plants caused the change of pod shape. A new high-density genetic map with total 3411 markers was constructed with SLAF-seq technique, and thus enabled the detection of narrow interval QTLs for relevant traits in grapevine. VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350 were found to be related to berry firmness, while VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200 were linked to berry shape.

中文翻译：

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基于鲜食葡萄高密度遗传连锁图谱构建的浆果品质性状新的稳定QTL鉴定。

香气，浆果硬度和浆果形状是鲜食葡萄生产中的三个主要品质特征，也是葡萄育种中重要的目标特征。但是，有关其遗传机制的信息有限，导致葡萄品质育种的准确性和效率较低。基于遗传连锁图的构建，对数量性状位点（QTL）进行定位和分离是破解复杂数量性状遗传决定因素的有效方法。在目前的工作中，使用特定长度扩增片段测序（SLAF-seq）技术生成了一个最终的整合图，该图由19个连锁组（LG）上的3411个SLAF标记组成，相邻标记之间的平均距离为0.98 cM。马斯喀特风味共有9个重要的稳定QTL，在2016年至2018年连续三年进行分析的杂种中，在两个连锁群中确定了浆果硬度和浆果形状。值得注意的是，首次在LG 8上首次发现了关于浆果硬度和浆果形状的新稳定QTL。根据主要QTL区域的候选基因的生物学功能和表达谱，将与浆果硬度相关的3个基因（VIT_08s0007g00440，VIT_08s0040g02740和VIT_08s0040g02350）和3个基因（VIT_08s0032g01110，VIT_08s0032g01150和VIT_08s0105g00200）关联起来。VIT_08s0032g01110在转基因拟南芥植物中的过表达引起荚果形状的变化。使用SLAF-seq技术构建了一个新的高密度遗传图谱，共包含3411个标记，从而能够检测出窄间隔的QTL，以鉴定葡萄的相关性状。