The excellent water solubility of hydrazine (N₂H₄) allows it to easily invade the human body through the skin and respiratory tract, thereby damaging human organs and the central nervous system. To realize the monitoring of N₂H₄ effectively, first, coumarin was used to construct an inner alicyclic ring as the reaction site, extending the conjugation and strengthening the rigidity of the probe Co-Hy to improve its luminescence performance and enhance its ability to resist acids and alkalis. Second, we introduced a carboxyl group at the ortho position of the inner alicyclic ring to improve the water solubility of Co-Hy, and its strong electron pulling effect increased the activity of the reaction site. Spectroscopy experiments showed that Co-Hy featured excellent water solubility, high pH resistance (pH 4–11), excellent selectivity, fast analysis speed (within 5 minutes), and a low detection limit toward N₂H₄ (69 nM, 2.2 ppb). In addition, test-strip, spray, and cell-imaging experiments confirmed the outstanding application potential of Co-Hy for convenient N₂H₄ analysis in a variety of environments.

中文翻译：

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实用的pH兼容荧光传感器，用于土壤，水和活细胞中的肼。

肼（N ₂ H ₄）的优异水溶性使其可以通过皮肤和呼吸道轻易侵入人体，从而损害人体器官和中枢神经系统。为了有效地实现对N ₂ H ₄的监测，首先，香豆素被用于构建一个内部脂环族环作为反应位点，从而扩展了Co-Hy探针的共轭性并增强了其刚性，从而提高了其发光性能并增强了其发光能力。抵抗酸和碱。其次，我们在内部脂环族环的邻位引入了一个羧基，以改善Co-Hy的水溶性，其强大的电子拉动作用提高了反应部位的活性。光谱实验表明，Co-Hy具有优异的水溶性，高pH耐性（pH 4-11），出色的选择性，快速的分析速度（5分钟以内）和对N ₂ H ₄的低检测限（69 nM，2.2 ppb） ）。此外，测试条，喷雾和细胞成像实验证实了Co-Hy在各种环境中方便进行N ₂ H ₄分析的巨大应用潜力。