Abstract

The study evaluates the effect of two traditional horticulture treatments mentioned in Vrikshayurveda, a text from ancient India on the science of plant life, namely Kunapa jala (KJ) and Pancha gavya (PG) on the production of Withaferin A (WFA), withanolide A (WIA) and Withanolide B (WIB) in Withania somnifera (L) Dunal. Leaves and roots of W. somnifera were collected from different treated groups viz. control, KJ, PG, farmyard manure (FYM) and inorganic fertilizer (NPK). Reverse phase ultra-flow liquid chromatography (RP-UFLC) method was developed, validated for simultaneous detection of WFA, WIA and WIB. Statistical analysis of data was performed by ANOVA and tested for significance by the Dunnett multiple comparison test and data were expressed as mean ± standard deviation (SD). Results revealed, leaves possessed highest WFA content and roots possessed highest content of WIA and WIB. PG treated leaves were observed highest WFA (18.29 mg/g) and roots were observed highest WIA (19.63 mg/g) and WIB (1.36 mg/g). Conclusively, RP-UFLC method for simultaneous detection of withanolides has been developed and validated to evaluate the effect of traditional horticulture treatments. It is concluded that the enhanced production of withanolides can be achieved by the application of PG when compared to NPK application.

中文翻译：

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传统的园艺实践在Withania Somnifera（L.）Dunal-A RP-UFLC分析中提高了某些Withanolides的产量。

摘要

该研究评估了印度古代文献Vrikshayurveda中提到的两种传统园艺处理对植物生命科学的影响，即Kunapa jala（KJ）和Pancha gavya（PG）对Withaferin A（WFA），withanolide A的生产的影响。 （WIA）和Withaniaide B（WIB）在Withania somnifera（L）Dunal。W. somnifera的叶子和根从不同治疗组收集。对照，KJ，PG，农家肥（FYM）和无机肥料（NPK）。开发了反相超流动液相色谱（RP-UFLC）方法，并已验证可同时检测WFA，WIA和WIB。数据的统计分析通过ANOVA进行，并通过Dunnett多重比较检验进行显着性检验，数据表示为均值±标准差（SD）。结果显示，叶片具有最高的WFA含量，根部具有最高的WIA和WIB含量。PG处理的叶片观察到最高WFA（18.29 mg / g），根部观察到最高WIA（19.63 mg / g）和WIB（1.36 mg / g）。最终，已经开发出并同时检测anol醇化物的RP-UFLC方法，以评估传统园艺疗法的效果。