The L.p.SG1 DETECT Kit is a rapid, quantitative method for the detection and enumeration of Legionella pneumophila serogroup 1 (L.p. SG1) bacteria from different water matrixes. The method is based on a combination of immunomagnetic separation (IMS) and flow cytometric (FCM) quantification. To this end, the method employs magnetic particles conjugated to anti-L.p. SG1 antibodies for the IMS of the target bacteria from environmental matrices and fluorescently labelled anti-L.p. SG1 antibodies for subsequent quantification by FCM. The IMS can be performed either manually with a magnetic rack (rqmicro.MIMS) or automated with the rqmicro.STREAM sample preparation instrument. Compared to the reference method ISO 11731:2017, which is based on culturing and enumeration of colony forming units (CFU) on agar plates, and can take up to 10 days until results are available, analysis with the L.p. SG1 DETECT Kit is culture-independent and delivers results within 2 h.

中文翻译：

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验证嗜肺军团菌SG1 DETECT试剂盒用于定量饮用水，工艺水和地表水中的嗜肺军团菌血清群1细菌：AOAC性能测试方法^SM 052002

所述的Lp SG1 DETECT试剂盒是用于检测和计数快速，定量方法嗜肺军团菌血清组1（脂蛋白SG1）从不同的水基质的细菌。该方法基于免疫磁分离（IMS）和流式细胞仪（FCM）定量的结合。为此，该方法使用与抗Lp SG1抗体缀合的磁性颗粒对来自环境基质的目标细菌进行IMS，并使用荧光标记的抗LpSG1抗体，用于随后通过FCM定量。IMS可以使用磁架（rqmicro.MIMS）手动执行，也可以使用rqmicro.STREAM样品制备仪器自动执行。与参考方法ISO 11731：2017相比，后者基于在琼脂平板上培养和计数菌落形成单位（CFU）的方法，并且可能需要长达10天的时间才能获得结果，使用Lp SG1 DETECT试剂盒进行的分析是独立并在2小时内提供结果。