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Plasmonically Enhanced Photoelectrochemical Immunoassay Based on Au Nanoparticle-Loaded PAMAM Dendrimers for Cry1Ab Protein Detection
ACS Applied Nano Materials ( IF 5.9 ) Pub Date : 2020-09-03 , DOI: 10.1021/acsanm.0c02031
Shuyun Meng 1 , Ningning Zhang 2 , Dong Liu 1 , Xiuli Shen 1 , Yuye Li 1 , Fan Jia 1 , Yang Yang 2 , Tianyan You 1
Affiliation  

The widespread introduction of Cry1Ab protein in genetically modified crops has aroused considerable concern about the risks on the environment and food safety. Herein, we reported a plasmonic enhanced photoelectrochemical (PEC) immunosensor for the precise determination of the Cry1Ab protein. Its working principle was based on the space confinement-induced surface plasmon resonance to enhance visible-light absorption of photoactive materials. The polyamidoamine (PAMAM) dendrimers were selected as the space-confined template to synthesize gold (Au) nanoparticles, that is, PAMAM–Au, which presented smaller diameter and good dispersive stability. Meanwhile, PAMAM–Au nanocomposites can exhibit significantly enhanced surface electric field intensity according to the finite-difference time-domain analysis. Further, the efficient PEC enhancement of PAMAM–Au was then validated by the highly increased photocurrent in CdSe/PAMAM–Au nanocomposites. The as-fabricated PEC immunosensor provided a wide linear range of over 4 orders of magnitude (0.01–100 ng mL–1), with the detection limit down to 3 pg mL–1 for Cry1Ab analysis. Besides, such an immunosensor was applied to analyze Cry1Ab in corn and wheat samples, and the result was in good agreement with that by the official method, enzyme-linked immunosorbent assay. Our work provides a way to fabricate high-performance sensors for the accurate detection of Cry1Ab in the environment and food products.

中文翻译:

基于金纳米颗粒的PAMAM树状大分子的等离子增强光电化学免疫检测Cry1Ab蛋白。

Cry1Ab蛋白在转基因作物中的广泛引入引起了人们对环境和食品安全风险的广泛关注。在本文中,我们报道了用于精确测定Cry1Ab蛋白的等离激元增强型光电化学(PEC)免疫传感器。它的工作原理是基于空间限制引起的表面等离子体激元共振,以增强光敏材料的可见光吸收。选择聚酰胺基胺(PAMAM)树状聚合物作为空间受限的模板,以合成金(Au)纳米粒子,即PAMAM–Au,它具有较小的直径和良好的分散稳定性。同时,根据有限差分时域分析,PAMAM–Au纳米复合材料可表现出明显增强的表面电场强度。进一步,然后通过CdSe / PAMAM-Au纳米复合材料中光电流的高度增加,验证了PAMAM-Au的有效PEC增强。预制的PEC免疫传感器可提供超过4个数量级的宽线性范围(0.01–100 ng mL–1),Cry1Ab分析的检测限低至3 pg mL –1。此外,这种免疫传感器被用于分析玉米和小麦样品中的Cry1Ab,其结果与官方方法酶联免疫吸附法测定的结果相吻合。我们的工作提供了一种制造高性能传感器的方法,用于准确检测环境和食品中的Cry1Ab。
更新日期:2020-09-25
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