Abstract Thermophilic bacteria have attracted great attention due to their ability to produce thermostable enzymes. The aim of this study was the isolation and characterization of thermophilic bacteria from Gavmesh Goli hot spring in Sareyn, North West of Iran. Of 10 water samples collected, 36 thermophilic bacteria were obtained. The thermophilic bacteria were tested for their ability to produce hydrolase enzymes. All the isolates were potentially protease producers. Lipase, DNase, and amylase activities were confirmed in 34 (94.4%), 8 (22.2%), and 3 (8.3%) isolates, respectively. Five isolates with higher levels of enzyme activity were selected for further studies. Morphological, biochemical, and molecular analysis by 16S rRNA gene sequencing revealed that four isolates (DH15, DH16, DH20, and DH29) could be identified as Thermomonas hydrothermalis and one (PA10) Bacillus altitudinis. The protease produced by these isolates was optimally active at 50–55 °C, pH 8–8.5, and 0–0.5 M NaCl. In this first time study, we isolated T. hydrothermalis and B. altitudinis from Iranian hot springs and demonstrated the characteristics of T. hydrothermalis protease. Accordingly, due to the valuable potential of these bacteria such as the production of protease with high temperature and pH stability, these isolates can be introduced as promising candidates for industrial applications.

中文翻译：

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伊朗 Sabalan 地热田 Gavmesh Goli 温泉嗜热细菌的分离和表征：Thermomonas hydrothermalis 和 Bacillus altitudinis 分离物作为热稳定蛋白酶的潜在来源

摘要 嗜热细菌因其产生耐热酶的能力而备受关注。本研究的目的是从伊朗西北部萨雷恩的 Gavmesh Goli 温泉中分离和表征嗜热细菌。在收集的 10 个水样中，获得了 36 个嗜热细菌。测试了嗜热细菌产生水解酶的能力。所有分离株都是潜在的蛋白酶生产者。分别在 34 个 (94.4%)、8 个 (22.2%) 和 3 个 (8.3%) 分离株中证实了脂肪酶、DNA 酶和淀粉酶活性。选择了五个酶活性水平较高的分离株进行进一步研究。通过 16S rRNA 基因测序进行的形态学、生化和分子分析揭示了四种分离株（DH15、DH16、DH20、和 DH29) 可以被鉴定为 Thermomonas hydrothermalis 和一种 (PA10) Bacillus altitudinis。这些分离物产生的蛋白酶在 50–55 °C、pH 8–8.5 和 0–0.5 M NaCl 下具有最佳活性。在这项首次研究中，我们从伊朗温泉中分离出了 T. hydrothermalis 和 B. altitudinis，并展示了 T. hydrothermalis 蛋白酶的特性。因此，由于这些细菌的宝贵潜力，例如生产具有高温和 pH 稳定性的蛋白酶，这些分离物可以作为工业应用的有希望的候选物引入。来自伊朗温泉的海拔高度，并展示了 T. hydrothermalis 蛋白酶的特性。因此，由于这些细菌的宝贵潜力，例如生产具有高温和 pH 稳定性的蛋白酶，这些分离物可以作为工业应用的有希望的候选物引入。来自伊朗温泉的海拔高度，并展示了 T. hydrothermalis 蛋白酶的特性。因此，由于这些细菌的宝贵潜力，例如生产具有高温和 pH 稳定性的蛋白酶，这些分离物可以作为工业应用的有希望的候选物引入。