当前位置: X-MOL 学术Pharm. Biol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Anti-proliferation and anti-migration effects of an aqueous extract of Cinnamomi ramulus on MH7A rheumatoid arthritis-derived fibroblast-like synoviocytes through induction of apoptosis, cell arrest and suppression of matrix metalloproteinase
Pharmaceutical Biology ( IF 3.8 ) Pub Date : 2020-01-01 , DOI: 10.1080/13880209.2020.1810287
Jia Liu 1, 2 , Qing Zhang 1 , Ruo-Lan Li 1 , Shu-Jun Wei 3 , Yong-Xiang Gao 3 , Li Ai 4 , Chun-Jie Wu 1 , Xu-Feng Pu 1, 2, 5
Affiliation  

CONTEXT Cinnamomi ramulus, the dry twig of Cinnamomum cassia Presl. (Lauraceae), has been reported to exert several activities such as antitumor, anti-inflammatory, and analgesic effects. OBJECTIVE This study investigates the effects of an aqueous extract of Cinnamomi ramulus (ACR) on rheumatoid arthritis (RA). MATERIALS AND METHODS TNF-α-induced RA-derived fibroblast-like synoviocyte MH7A cells were incubated with ACR (0.1-2 mg/mL) for 24 h. The proliferation was tested using CCK-8 and colony formation assays. The migration and invasion abilities were measured using transwell tests and wound healing assays. Apoptosis and cell cycle were examined by flow cytometry. The potential mechanisms were determined by western blotting and quantitative real-time PCR. UPLC-QE-MS/MS was used for chromatographic analysis of ACR and its compounds were identified. Molecular docking strategy was used to screen the potential anti-RA active compounds of ACR. RESULTS We found that ACR induced apoptosis in MH7A cells at concentrations of 0.4, 0.8, and 1.2 mg/mL. The proliferation of MH7A cells was reduced and the cell cycle was blocked in the G2/M phase at concentrations of 0.2, 0.4, 0.6 mg/mL. Migration and invasion of MH7A cells were reduced through inhibiting the expression of MMP-1, MMP-2, and MMP-3. The molecular docking strategy results showed that 9 compounds in ACR have good affinity with protein crystal, and benzyl cinnamate (10-100 µg/mL) could inhibit cell migration and induce apoptosis. CONCLUSIONS The anti-RA effect of ACR may be attributed to its anti-proliferative and anti-migration effects on synovial fibroblasts. These data suggest that Cinnamomi ramulus may have therapeutic value for the treatment of RA.

中文翻译:

肉桂水提物通过诱导细胞凋亡、细胞停滞和抑制基质金属蛋白酶对 MH7A 类风湿性关节炎衍生的成纤维细胞样滑膜细胞的抗增殖和抗迁移作用

背景 Cinnamomi ramulus,Cinnamomum cassia Presl 的干燥枝条。据报道,(Lauraceae)具有多种活性,例如抗肿瘤、抗炎和镇痛作用。目的 本研究探讨肉桂枝水提物 (ACR) 对类风湿性关节炎 (RA) 的影响。材料与方法TNF-α诱导的RA衍生的成纤维样滑膜细胞MH7A细胞与ACR(0.1-2 mg/mL)一起孵育24 h。使用 CCK-8 和集落形成测定法测试增殖。使用transwell测试和伤口愈合测定测量迁移和侵袭能力。通过流式细胞术检查细胞凋亡和细胞周期。通过蛋白质印迹和定量实时PCR确定潜在机制。UPLC-QE-MS/MS 用于 ACR 的色谱分析并鉴定了其化合物。采用分子对接策略筛选ACR潜在的抗RA活性化合物。结果 我们发现 ACR 在 0.4、0.8 和 1.2 mg/mL 的浓度下诱导 MH7A 细胞凋亡。在0.2、0.4、0.6 mg/mL的浓度下,MH7A细胞的增殖减少,细胞周期在G2/M期被阻断。通过抑制MMP-1、MMP-2和MMP-3的表达来减少MH7A细胞的迁移和侵袭。分子对接策略结果表明,ACR中的9种化合物与蛋白质晶体具有良好的亲和力,肉桂酸苄酯(10-100 µg/mL)可抑制细胞迁移并诱导细胞凋亡。结论 ACR的抗RA作用可能与其对滑膜成纤维细胞的抗增殖和抗迁移作用有关。
更新日期:2020-01-01
down
wechat
bug