The objective of this study was to evaluate the Micronaut-S carbapenemase detection microtiter plate assay for the detection of carbapenemases and Ambler class determination. The Micronaut-S carbapenemase detection microtiter plate was tested using a challenging collection of 154 carbapenemase-producing and 150 carbapenemase-negative clinical strains of Enterobacterales and Pseudomonas aeruginosa. The Micronaut-S carbapenemase detection assay was able to detect 148/154 carbapenemase producers correctly, whereas 5/150 non-carbapenemase-producing isolates tested as false positive. This resulted in an overall sensitivity of 96% and a specificity of 97%. Regarding the detection of the carbapenemase class, the sensitivities and specificities were 93%/100%, 96%/100%, and 97%/99% for class A (n = 27), class B (n = 54), and class D (n = 73) carbapenemases, respectively. The Micronaut-S carbapenemase detection microtiter plate represents an easy-to-use and valuable tool for accurate and reliable detection of carbapenemases. In addition, it provides identification of the class of carbapenemase in most cases which can provide significant therapy guidance.

中文翻译：

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通过高度专业化的MICRONAUT-S微稀释测定法检测肠杆菌和铜绿假单胞菌中碳青霉烯酶类别（包括OXA-48-like酶）的表型。

这项研究的目的是评估Micronaut-S碳青霉烯酶检测微量滴定板检测碳青霉烯酶的检测和Ambler类的确定。Micronaut-S碳青霉酶检测微量滴定板使用了挑战性的154种产碳青霉酶和150种碳青霉烯酶阴性的肠杆菌和铜绿假单胞菌临床菌株进行了测试。Micronaut-S碳青霉烯酶检测方法能够正确检测148/154个碳青霉烯酶的产生者，而5/150个非产生碳青霉烯酶的分离株则被测试为假阳性。这导致总体敏感性为96％，特异性为97％。关于碳青霉烯酶类别的检测，A类（n = 27），B类（n = 54）和Class的敏感性和特异性分别为93％/ 100％，96％/ 100％和97％/ 99％。D（n = 73）碳青霉烯酶。Micronaut-S碳青霉烯酶检测微量滴定板代表了一种易于使用且有价值的工具，可用于准确，可靠地检测碳青霉烯酶。另外，在大多数情况下，它可以鉴定碳青霉烯酶的类别，从而可以提供重要的治疗指导。