Excessive exposure to Copper (Cu) may result in Cu toxicity and adversely affect health outcomes. We investigated the protective role of rutin on Cu‐induced brain damage. Experimental rats were treated as follows: group I: control; group II: Cu‐sulfate: 200 mg/kg; group III: Cu‐sulfate, and rutin 100 mg/kg; and group IV: rutin 100 mg/kg, for 7 weeks. Cu only treatment significantly decreased body weight gain, while rutin cotreatment reversed this decrease. Cu treatment increased malondialdehyde, nitric oxide level, and myeloperoxidase activity and decreased superoxide dismutase and catalase activities in rat brain. Immunohistochemistry showed that COX‐2, iNOS, and Bcl‐2 proteins were strongly expressed, while Bax was mildly expressed in the brain of Cu‐treated rats. Furthermore, brain histology revealed degenerated neurons, and perforated laminae of cerebral cortex in the Cu‐only treated rats. Interestingly, coadministration of Cu and rutin reduced the observed histological alteration, improved inflammatory and antioxidant biomarkers, thereby protecting against Cu‐induced brain damage via antioxidative and anti‐inflammatory mechanisms.

中文翻译：

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芦丁通过抗氧化和消炎作用改善了硫酸铜诱导的脑损伤。

过量暴露于铜（Cu）可能会导致Cu毒性并对健康结果产生不利影响。我们研究了芦丁对铜诱导的脑损伤的保护作用。实验大鼠的治疗如下：第一组：对照组；第二组：对照组。第II组：硫酸铜：200 mg / kg；第三组：硫酸铜和芦丁100 mg / kg; 第四组：芦丁100 mg / kg，持续7周。仅铜治疗显着降低了体重增加，而芦丁共治疗则逆转了这种下降。铜处理可增加大鼠脑中的丙二醛，一氧化氮水平和髓过氧化物酶活性，并降低超氧化物歧化酶和过氧化氢酶活性。免疫组织化学显示，在Cu处理过的大鼠的大脑中，COX-2，iNOS和Bcl-2蛋白表达强烈，而Bax轻度表达。此外，脑组织学检查显示神经元退化，仅用铜处理的大鼠大脑皮层的穿孔和穿孔。有趣的是，铜和芦丁的共同给药减少了观察到的组织学改变，改善了炎症和抗氧化生物标志物，从而通过抗氧化和抗炎机制防止铜诱导的脑损伤。