The hypoxic microenvironment can facilitate the tumor progression, and transcription factor YY1 holds promoting effects in various tumors. This work aims to investigate whether YY1 is involved in hypoxia‐induced stemness of lung cancer cells. We showed that hypoxic microenvironment induced the expression of HIF‐1α and YY1, and the stemness of lung cancer cells, which was attenuated by YY1 knockdown. Additionally, we found that YY1 regulates the hypoxia‐induced stemness in a HIF‐1α‐dependent manner, but independent on p53 expression. Further analysis revealed that YY1 physically interacted with HIF‐1α protein and stabilized HIF‐1α protein. Our work indicates a novel YY1/HIF‐1α axis regulating the stemness of lung cancer cells.

中文翻译：

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转录因子YY1通过在缺氧微环境下稳定缺氧因子HIF ‐1α增强肺癌细胞的干性

缺氧微环境有利于肿瘤进展，转录因子YY1对多种肿瘤具有促进作用。本工作旨在探讨YY1是否参与缺氧诱导的肺癌细胞干细胞性。我们发现，低氧微环境诱导 HIF-1α 和 YY1 的表达，以及肺癌细胞的干性，而 YY1 敲低则减弱了这种干性。此外，我们发现 YY1 以 HIF-1α 依赖性方式调节缺氧诱导的干性，但不依赖于 p53 表达。进一步分析表明，YY1 与 HIF-1α 蛋白发生物理相互作用，并稳定了 HIF-1α 蛋白。我们的工作表明了一种新的 YY1/HIF-1α 轴调节肺癌细胞的干性。