FCS 3.2 is a revision of the flow cytometry data standard based on a decade of suggested improvements from the community as well as industry needs to capture instrument conditions and measurement features more precisely. The unchanged goal of the standard is to provide a uniform file format that allows files created by one type of acquisition hardware and software to be analyzed by any other type. The standard retains the overall FCS file structure and most features of previous versions, but also contains a few changes that were required to support new types of data and use cases efficiently. These changes are incompatible with existing FCS file readers. Notably, FCS 3.2 supports mixed data types to, for example, allow FCS measurements that are intrinsically integers (e.g., indices or class assignments) or measurements that are commonly captured as integers (e.g., time ticks) to be more represented as integer values, while capturing other measurements as floating‐point values in the same FCS data set. In addition, keywords explicitly specifying dyes, detectors, and analytes were added to avoid having to extract those heuristically and unreliably from measurement names. Types of measurements were formalized, several keywords added, others removed, or deprecated, and various aspects of the specification were clarified. A reference implementation of the cyclic redundancy check (CRC) calculation is provided in two programming languages since a correct CRC implementation was problematic for many vendors. © 2020 International Society for Advancement of Cytometry

中文翻译：

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流式细胞术数据文件标准，版本 FCS 3.2。

FCS 3.2 是流式细胞术数据标准的修订版，基于社区十年来的建议改进以及行业需求，以更精确地捕获仪器条件和测量功能。该标准不变的目标是提供统一的文件格式，允许任何其他类型的采集硬件和软件对由一种类型的采集硬件和软件创建的文件进行分析。该标准保留了整个 FCS 文件结构和以前版本的大多数功能，但也包含有效支持新类型数据和用例所需的一些更改。这些更改与现有的 FCS 文件读取器不兼容。值得注意的是，FCS 3.2 支持混合数据类型，例如允许本质上是整数的 FCS 测量值（例如索引或类分配）或通常捕获为整数（例如时间刻度）的测量值更多地表示为整数值。同时在同一 FCS 数据集中将其他测量值捕获为浮点值。此外，还添加了明确指定染料、检测器和分析物的关键字，以避免必须从测量名称中启发式且不可靠地提取这些内容。测量类型被正式化，添加了几个关键字，删除或弃用了其他关键字，并且澄清了规范的各个方面。由于正确的 CRC 实现对许多供应商来说是个问题，因此以两种编程语言提供了循环冗余校验 (CRC) 计算的参考实现。© 2020 国际细胞计数促进会