Importance of enzymes is ever-rising particularly microbial lipases holding great industrial worth owing to their potential to catalyze a diverse array of chemical reactions in aqueous as well as nonaqueous settings. International lipase market is anticipated to cross USD 797.7 million till 2025, rising at a 6.2% compound annual growth rate from 2017 to 2025. The recent breakthrough in the field of lipase research is the generation of new and upgraded versions of lipases via molecular strategies. For example, integration of rational enzyme design and directed enzyme evolution to attain desired properties in lipases. Normally, purification of lipase with significant purity is achieved through a multistep procedure. Such multiple step approach of lipase purification entails both conventional and novel techniques. The present review attempts to provide an overview of different aspects of lipase production including fermentation techniques, factors affecting lipase production, and purification strategies, with the aim to assist researchers to pick a suitable technique for the production and purification of lipase.

中文翻译：

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微生物脂肪酶生产：深入了解脂肪酶生产和纯化技术的最新进展

酶的重要性不断上升，尤其是微生物脂肪酶，因为它们具有在水性和非水性环境中催化各种化学反应的潜力，因此具有巨大的工业价值。预计到 2025 年国际脂肪酶市场将超过 7.977 亿美元，从 2017 年到 2025 年以 6.2% 的复合年增长率增长。 脂肪酶研究领域的最新突破是通过分子策略产生新的和升级版本的脂肪酶。例如，整合合理的酶设计和定向酶进化以获得脂肪酶所需的特性。通常，具有显着纯度的脂肪酶的纯化是通过多步骤程序实现的。这种脂肪酶纯化的多步骤方法需要传统和新技术。