The enzymatic oxidation of glucose to produce reactive oxygen species (ROS) provides honey with antimicrobial efficacy. This mechanism offers an alternative to traditional antibiotics, however topical use of honey is limited due to its adherent and highly viscous properties. This study aims to overcome these issues by engineering a powder-based system that eases delivery and offers in situ activation of ROS.

Starch based drying agents were utilised to enable freeze drying of a medical honey, with methylated-β-cyclodextrin (MCD) enabling the highest active incorporation (70%) whilst still producing a free-flowing powder. Addition of a superabsorbent, sodium polyacrylate (≤40%) was shown to facilitate in situ gelation of the powder, with an absorption capacity of up to 120.7 ±4.5 mL g^-1.

Promisingly efficacy of the optimised superabsorbent powder was demonstrated in vitro against several clinically relevant Gram-negative and Gram–positive bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa). Alongside this no adverse effects were observed against human dermal fibroblasts. Application of the superabsorbent powder in an ex-vivo porcine wound model revealed capability to form a protective hydrogel barrier in less than 1 minute. Overall this novel ROS producing superabsorbent powder has potential to tackle topical infections without using traditional antibiotics.

葡萄糖的酶促氧化产生活性氧（ROS）为蜂蜜提供了抗菌功效。该机制提供了传统抗生素的替代方法，但是由于其粘附性和高粘性，蜂蜜的局部使用受到限制。这项研究旨在通过设计一种基于粉末的系统来克服这些问题，该系统易于输送并提供ROS的原位活化。

淀粉基干燥剂用于使医用蜂蜜冷冻干燥，而甲基化的β-环糊精（MCD）可实现最高的活性掺入（70％），同时仍可产生自由流动的粉末。显示出添加超吸收性聚丙烯酸钠（≤40％）有助于粉末的原位凝胶化，吸收容量高达120.7±4.5 mL g ^-1。

在体外证明了优化的超吸收性粉末对几种临床相关革兰氏阴性和革兰氏阳性细菌（金黄色葡萄球菌，大肠杆菌和铜绿假单胞菌）的功效。除此之外，未观察到对人皮肤成纤维细胞的不利影响。超吸收性粉末在离体猪伤口模型中的应用显示了在不到1分钟的时间内形成保护性水凝胶屏障的能力。总体而言，这种新型的产生ROS的超吸收性粉末具有在不使用传统抗生素的情况下解决局部感染的潜力。