Biomaterials Advances ( IF 7.9 ) Pub Date : 2020-09-03 , DOI: 10.1016/j.msec.2020.111473 Irina Yu Zhuravleva , Elena V. Karpova , Ludmila A. Oparina , Olga V. Poveschenko , Maria A. Surovtseva , Anatoly T. Titov , Alexander L. Ksenofontov , Maria B. Vasilieva , Elena V. Kuznetsova , Alexander V. Bogachev-Prokophiev , Boris A. Trofimov
Bioprosthetic heart valves made from bovine pericardium (BP) and porcine pericardium (PP) preserved with glutaraldehyde (GA) are commonly used in valve surgeries but prone to calcification in many patients. In this study, we compared BP and PP preserved with GA, ethylene glycol diglycidyl ether (DE), and 1,2,3,4,6-penta-O-{1-[2-(glycidyloxy)ethoxy]ethyl}-D-glucopyranose (PE). We studied the stabilities of DE and PE in preservation media along with the amino acid (AA) compositions, Fourier-transform infrared spectra, mechanical properties, surface morphologies, thermal stability, calcification, and the cytocompatibility of BP and PP treated with 0.625% GA, 5% DE, 2% PE, and alternating 5% DE and 2% PE for 3 +11 d and 10 + 10 d, respectively. Both epoxides were stable in the water-buffer solutions (pH 7.4). DE provided high linkage densities in BP and PP owing to reactions with Hyl, Lys, His, Arg, Ser, and Tyr. PE reacted weakly with these AAs but strongly with Met. High cross-linking density obtained using the 10 d + 10 d method provided satisfactory thermal stability of biomaterials. The epoxy preservations improved cytocompatibility and resistance to calcification. PE enhanced the stress/strain properties of the xenogeneic pericardia, perhaps by forming nanostructures that were clearly visualised in BP using scanning electron microscopy. The DE + PE combination, in an alternating cross-linking manner, thus constitutes a promising option for developing bioprosthetic pericardia.
中文翻译:
使用五环氧化物改善牛和猪生物假体心包的交联方法:多参数评估研究
由牛心包(BP)和猪心包(PP)制成的生物假体心脏瓣膜保存有戊二醛(GA),通常用于瓣膜手术,但在许多患者中易于钙化。在这项研究中,我们比较了GA,乙二醇二缩水甘油醚(DE)和1,2,3,4,6-penta- O-保留的BP和PP{1- [2-(缩水甘油基氧基)乙氧基]乙基} -D-吡喃葡萄糖(PE)。我们研究了DE和PE在保存介质中的稳定性以及氨基酸(AA)组成,傅立叶变换红外光谱,机械性能,表面形态,热稳定性,钙化以及用0.625%GA处理的BP和PP的细胞相容性,5%的DE,2%的PE和5%的DE和2%的PE分别持续3 +11 d和10 + 10 d。两种环氧化物在水缓冲溶液(pH 7.4)中均稳定。由于与Hyl,Lys,His,Arg,Ser和Tyr的反应,DE在BP和PP中提供了高连接密度。PE对这些AA的反应较弱,但对Met的反应强烈。使用10 d + 10 d方法获得的高交联密度为生物材料提供了令人满意的热稳定性。环氧防腐剂改善了细胞相容性和抗钙化性。PE可能通过形成纳米结构而增强了异种心包心包的应力/应变特性,这可以使用扫描电子显微镜在BP中清楚地看到。因此,DE + PE组合以交替交联的方式构成了开发生物假体心包的有前途的选择。