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Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA.
Journal of Virological Methods ( IF 3.1 ) Pub Date : 2020-09-03 , DOI: 10.1016/j.jviromet.2020.113965
Detlef Michel 1 , Karin M Danzer 2 , Rüdiger Groß 3 , Carina Conzelmann 3 , Janis A Müller 3 , Axel Freischmidt 2 , Jochen H Weishaupt 2 , Sandra Heller 4 , Jan Münch 3 , Manuela Michel 1 , Thomas Stamminger 1 , Alexander Kleger 4 , Markus Otto 2
Affiliation  

Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capacities delivering results in a rapid and reliable manner are a prerequisite. Here, we provide and validate such a rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA, termed COVID-quick-DET. This straightforward method operates with simple proteinase K treatment and repetitive heating steps with a sensitivity of 94.6% in head-to-head comparisons with kit-based isolation methods. This result is supported by data obtained from serially diluted SARS-CoV-2 virus stocks. Given its cost- and time-effective operation, COVID-quick-DET might be best suited for countries with general shortage or temporary acute scarcity of resources and equipment.



中文翻译:

快速、方便和高效的 SARS-CoV-2 RNA 独立试剂盒检测。

大流行性 SARS-CoV-2 感染已迅速发展成为一场社会经济和人道主义灾难。防止 SARS-CoV-2 传播的基本原则是保持社交距离、戴口罩、接触者追踪和及早发现 SARS-CoV-2。为了满足这些要求,几乎无限的测试能力以快速可靠的方式提供结果是先决条件。在这里,我们提供并验证了这种快速、方便和高效的 SARS-CoV-2 RNA 独立试剂盒检测,称为 COVID-quick-DET。这种直接的方法采用简单的蛋白酶 K 处理和重复加热步骤,与基于试剂盒的分离方法进行头对头比较时,灵敏度为 94.6%。从连续稀释的 SARS-CoV-2 病毒库中获得的数据支持了这一结果。鉴于其成本和时间效益的运作,

更新日期:2020-10-02
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