This work aims to demonstrate a new high-efficient way to lignin degradation plus polyphenol enrichment in poplar bark sawdust by a co-fungal treatment with white rot fungi of C. subvermispora, C. cinereus and P. ostreatus in an equal volume-ratio fermentation for three weeks. The results indicated that compared with any one of these fungi, over 50 % lignin-degradation and 6-fold polyphenol enrichment were achieved at the 12th day for the best performance by these mixed-fungi treatments. The polyphenol enrichments from the increasing amount of protocatechuic acid and caffeic acid-3-glucoside were confirmed by HPLC-ESI-QTOF-MS/MS and ATR-FT-IR, which facilitated the lignin-degradation in turn. They were potentially ascribed to an obvious improvement activity of ligninolytic enzyme (laccase, lignin peroxidase and manganese-dependent peroxidase) for the lignin-degradation and β-glycosidase action for flavonoid degradation, respectively. All above-mentioned results will provide a potential protocol for lignin degradation and polyphenol enrichment when the different fungi in co-culture play a role in synergism.

这项工作的目的是通过白腐菌共同真菌治疗展示一个新的高效的方式来降解木质素加上杨树皮木屑多酚富集C. subvermispora，灰C.和平菇在等体积比例的发酵中发酵三周。结果表明，与这些真菌中的任何一种相比，这些混合真菌处理在第12天木质素的降解率均超过50％，而多酚的富集率达到6倍。HPLC-ESI-QTOF-MS / MS和ATR-FT-IR证实了原儿茶酸和咖啡酸-3-葡糖苷含量的增加使多酚富集，从而促进了木质素的降解。它们可能归因于木质素分解酶（漆酶，木质素过氧化物酶和锰依赖性过氧化物酶）对木质素降解和β的明显改善活性。-糖苷酶分别作用于类黄酮降解。当共培养中的不同真菌在协同作用中起作用时，所有上述结果将为木质素降解和多酚富集提供潜在的方案。