Abstract

We used conducting redox-active polymers based on chitosan and bovine serum albumin (BSA), covalently bound to mediators ferrocene or Neutral Red, or thionine, modified by carbon nanotubes, as a base of an amperometric biosensor for determining glucose. The structure of the obtained polymers was studied by IR spectroscopy and scanning electron microscopy. Their electrochemical characteristics were studied by cyclic voltammetry. The redox-active polymer based on BSA covalently bound to ferrocene and modified by carboxylated carbon nanotubes demonstrated the most promising characteristics. The biosensor based on the selected hybrid polymer is characterized by high sensitivity, a lower limit of determined concentrations of 0.05 mM, and a high correlation coefficient (R = 0.9919) with the results of determination of glucose in human blood by an alternative method.

中文翻译：

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基于牛血清白蛋白，二茂铁，羧化碳纳米管和葡萄糖氧化酶的混合氧化还原活性聚合物

摘要

我们使用基于壳聚糖和牛血清白蛋白（BSA）的氧化还原活性聚合物，共价键合到由碳纳米管修饰的二茂铁或中性红或亚硫氨酸，作为测定葡萄糖的安培生物传感器的基础。通过红外光谱和扫描电子显微镜研究获得的聚合物的结构。通过循环伏安法研究了它们的电化学特性。基于BSA的氧化还原活性聚合物与二茂铁共价结合并被羧化碳纳米管改性，表现出最有希望的特性。基于所选杂化聚合物的生物传感器的特点是灵敏度高，测定浓度的下限为0.05 mM，相关系数高（R = 0.9919），并通过另一种方法测定人血中的葡萄糖。