Abstract

In the present study a high performance liquid chromatographic–mass spectrometric (LC–MS) method was developed for the measurement of ascorbic acid in human plasma and white blood cells. Special attention was paid to the known difficulties of ascorbic acid stability, and a plasma sample preparation method was developed, accordingly. The novel objective of this work was to focus on the determination of plasma ascorbic acid concentrations considering the necessity of accurate determination for further clinical studies related to scurvy induced by various pathological conditions. The presented study describes in detail a validated LC–MS method with adequate accuracy and precision for quantitative determination of ascorbic acid in human plasma. The method was tested on human plasma and leukocyte samples.

中文翻译：

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高效液相色谱-质谱联用法定量血浆和白细胞中的维生素C

摘要

在本研究中，开发了一种用于测定人血浆和白细胞中抗坏血酸的高效液相色谱质谱法（LC-MS）。特别注意抗坏血酸稳定性的已知困难，因此开发了血浆样品制备方法。这项工作的新目标是集中在血浆抗坏血酸浓度的测定上，考虑到需要准确测定与各种病理条件引起的坏血病相关的进一步临床研究的必要性。本研究详细描述了一种经过验证的LC-MS方法，该方法具有足够的准确度和精密度，用于定量测定人血浆中的抗坏血酸。该方法在人血浆和白细胞样品上进行了测试。