Abstract

A novel molecularly imprinted solid–phase extraction method was developed coupled with HPLC for ceftazidime determination in biological fluids. Various parameters such as molecular recognition properties, adsorption capacity and selectivity of the molecularly imprinted polymer (MIP) were evaluated. The optimal conditions for solid-phase extraction consisted of conditioning with 5 mL of methanol and 20 mL of deionized water at neutral pH, aqueous sample loading (50 mL at pH 3.0), washing using 5 mL of acetone and elution with 5 mL of acetonitrile–water solution (2 : 8, v/v). The MIP selectivity was evaluated by checking several substances with molecular structures similar to that of ceftazidime. The MIP relative standard deviation was 4.1% (n = 5). The limits of detection and quantification for ceftazidime were 7 and 25 μg/L, respectively, while linearity range was 25–800 μg/L.

中文翻译：

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分子印迹聚合物的合成及其在固相萃取吸附剂测定人血清和尿液样品中头孢他啶的应用

摘要

一种新型的分子印迹固相萃取方法与HPLC相结合，用于测定生物液体中头孢他啶的含量。评估了各种参数，如分子识别性能，分子印迹聚合物（MIP）的吸附能力和选择性。固相萃取的最佳条件包括：在中性pH值下用5 mL甲醇和20 mL去离子水进行条件处理，加水样品（pH值为3.0时为50 mL），用5 mL丙酮洗涤并用5 mL乙腈洗脱–水溶液（2：8，v / v）。通过检查几种分子结构类似于头孢他啶的物质来评估MIP选择性。MIP相对标准偏差为4.1％（n= 5）。头孢他啶的检出限和定量限分别为7和25μg/ L，线性范围为25–800μg/ L。