当前位置: X-MOL 学术Plant Reprod. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Identification of pollen and pistil polygalacturonases in Nicotiana tabacum and their function in interspecific stigma compatibility.
Plant Reproduction ( IF 3.4 ) Pub Date : 2020-09-03 , DOI: 10.1007/s00497-020-00393-x
Jugou Liao 1 , Zhiyun Chen 1 , Xuemei Wei 1 , Keliang Tao 1 , Jingwen Zhang 1 , Xiaojun Qin 1 , Zihui Pan 1 , Wenguang Ma 2 , Lei Pan 3 , Shuai Yang 3 , Mingqiao Wang 4 , Xiaokun Ou 1 , Suiyun Chen 1
Affiliation  

Key message

The pollen and pistil polygalacturonases in Nicotiana tabacum were identified and found to regulate pollen tube growth and interspecific compatibility.

Abstract

Polygalacturonase (PG) is one of the enzymes catalyzing the hydrolysis of pectin. This process plays important roles in the pollen and pistil. In this research, the pollen and pistil PGs in Nicotiana tabacum (NtPGs) were identified, and their expression, localization and the potential function in the pollen and interspecific stigma incompatibility were explored. The results showed that 118 NtPGs were retrieved from the genome of N. tabacum. The phylogenetic tree and RT-qPCR analysis led to the identification of 10 pollen PGs; among them, two, seven and one showed specifically higher expression levels in the early development of anthers, during pollen maturation and in mature anthers, respectively, indicating their function difference. Immunofluorescence analysis showed that PGs were located in the cytoplasm of (1) mature pollen and (2) in vitro grown pollen tubes, as well as in the wall of in vivo grown pollen tubes. Four NtPGs in clade A were identified as the pistil PGs, and the pistil PGs were not found in clade E. Significantly higher PGs expression was recorded after incompatible pollination in comparison with the compatible stigma, indicating a potential function of PGs in regulating stigma incompatibility. The influence of PGs on pollen tube growth was explored in vitro and partly in vivo, showing that high PGs activity inhibited pollen tube growth. The application of PGs on the otherwise compatible stigma resulted in pollen tube growth inhibition or failure of germination. These results further supported that increased PGs expression in incompatible stigma might be partially responsible for the interspecific stigma incompatibility in Nicotiana.



中文翻译:

烟草中花粉和雌蕊多聚半乳糖醛酸酶的鉴定及其在种间柱头相容性中的作用。

关键信息

鉴定了普通烟草中的花粉和雌蕊多聚半乳糖醛酸酶,发现它们可以调节花粉管的生长和种间相容性。

抽象的

多聚半乳糖醛酸酶(PG)是催化果胶水解的酶之一。这个过程在花粉和雌蕊中起着重要作用。本研究对烟草中的花粉和雌蕊PGs (NtPGs) 进行了鉴定,并探讨了它们在花粉和种间柱头不亲和性中的表达、定位和潜在功能。结果表明,从普通烟草基因组中检索到 118 个NtPG. 系统发育树和 RT-qPCR 分析导致鉴定出 10 个花粉 PG;其中,2个、7个和1个分别在花药发育早期、花粉成熟期和成熟花药中表现出特异性较高的表达水平,表明它们的功能存在差异。免疫荧光分析表明,PGs 位于 (1) 成熟花粉和 (2) 体外生长的花粉管的细胞质中,以及体内生长的花粉管壁中。进化枝A中的4个NtPGs被鉴定为雌蕊PGs,而进化枝E中没有发现雌蕊PGs。与亲和的柱头相比,不亲和授粉后记录的PGs表达显着升高,表明PGs在调节柱头不亲和中的潜在功能。在体外和部分体内研究了 PGs 对花粉管生长的影响,表明高 PGs 活性抑制了花粉管的生长。PGs 在其他相容的柱头上的应用导致花粉管生长抑制或发芽失败。这些结果进一步支持了不相容柱头中 PGs 表达的增加可能是造成种间柱头不相容的部分原因。烟草

更新日期:2020-09-03
down
wechat
bug