Cytotoxicity of CuO nanoparticles (NPs) are an impediment in utilizing them as an effective nanocarriers of chemotherapeutic drugs for targeted drug delivery in nasopharyngeal cancer. In our current study, we have designed a two-step synthesis and coating of CuO NPs with different concentrations of PLGA (polylactide-co-glycolide) to reduce the cytotoxicity. This was further conjugated with folic acid to enhance targeting to specific tissue. The multiple drugs loaded in the NPs were two potent anticancer drugs doxorubicin and docetaxel. A complete characterization studies including micrographic analysis, zeta potential measurements, polydispersity index, Fourier transform infrared spectroscopy (FTIR), encapsulation and loading efficiencies, stability and in vitro release studies were done. Cytoxicity studies were done with MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, acridine orange/ethidium bromide and DAPI (4, 6-diamidino-2-phenylindole, dihydrochloride) staining procedures. Impediametric studies were also carried out to reinforce the reduction in cytotoxicity. Finally the cellular uptake of the NPs was seen. It was evident from the results that the multiple drugs loaded CuO NPs formed with PLGA coating were uniform, non-agglomerated in size ranging from 180 to 195 nm. The FTIR revealed no major changes in drug peaks. Encapsulation and loading efficiencies showed sufficient amount of drug being loaded into the NPs. The drug loaded NPs showed no change in size or zeta potential even after a period of 30 days. The cytotoxicity studies revealed significant reduction in toxicity after coating the surface treated with PLGA as evident from the microscopic analysis of cells. Hence the current study may be prioritized and further in vivo/in vitro studies may be carried out.

中文翻译：

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PLGA包覆的叶酸介导的载有多种药物的氧化铜（CuO）纳米颗粒的生物合成，表征及其对鼻咽癌细胞系的细胞毒性。

CuO纳米颗粒（NPs）的细胞毒性是将其用作鼻咽癌靶向药物的有效治疗药物纳米载体的障碍。在我们目前的研究中，我们设计了两步合成和包覆不同浓度PLGA（聚丙交酯-共-乙交酯）的CuO NP的方法，以降低细胞毒性。将其进一步与叶酸缀合以增强对特定组织的靶向。NP中装载的多种药物是两种有效的抗癌药阿霉素和多西他赛。完成了完整的表征研究，包括显微分析，ζ电势测量，多分散指数，傅里叶变换红外光谱（FTIR），包囊和负载效率，稳定性和体外释放研究。用MTT 3-（4，5-二甲基噻唑-2-基）-2,5-二苯基溴化四溴化铵测定，a啶橙/溴化乙锭和DAPI（4，6-二mid基-2-苯基吲哚，二盐酸盐）染色程序。还进行了阻抗学研究，以增强细胞毒性的降低。最后，看到了NP的细胞吸收。从结果可以明显看出，用PLGA涂层形成的载有多种药物的CuO NPs是均匀的，无团聚的，粒径范围为180至195 nm。FTIR显示药物峰没有重大变化。封装和装载效率表明有足够数量的药物被装载到NP中。即使在30天后，载有药物的NP的大小或Zeta电位也没有变化。细胞毒性研究表明，用PLGA处理的表面覆盖后，毒性显着降低，这从细胞的显微镜分析可以明显看出。因此，当前的研究可能会被优先考虑并进一步在体内/可以进行体外研究。