Acute lung injury (ALI) is a disorder of pulmonary tissue caused by prolonged inflammation and uncontrolled oxidative stress. Tilianin is a natural polyphenol, acknowledged for its anti-inflammatory and antioxidant activities. The goal of this work was to explore the effect of tilianin in an LPS-challenged ALI model. The mechanistic investigation was carried out using both in vitro and in vivo experiments. The RAW264.7 macrophage cell and C57BL/6 mice were treated with Tilianin (10 and 20 μM) and then induced with LPS (1 μg/mL) overnight. The C57BL/6 mouse was administered LPS intratracheally (2 mg/kg) to induce ALI prior to tilianin intraperitoneal treatment (10 mg/kg). In vitro, the results showed that Tilianin reduced secretion of LPS-induced proinflammatory cytokine. In vivo, prophylactic treatment with tilianin attenuated infiltration of macrophages and histopathological changes and improved inflammation as shown by a marked reduction in inflammatory mediators found in bronchoalveolar lavage fluid (BALF). Furthermore, tilianin effectively suppressed MDA levels and GSH and SOD in ALI were increased. Collectively, these findings suggest that tilianin is a potential agent for ameliorating LPS-induced ALI.

中文翻译：

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在针对急性肺损伤Tilianin的作用在体外LPS诱导的肺泡巨噬细胞的细胞和体内C57BL / 6小鼠模型

急性肺损伤（ALI）是由长期炎症和不受控制的氧化应激引起的肺组织疾病。Tilianin是一种天然多酚，以其抗炎和抗氧化作用而闻名。这项工作的目的是探索tilianin在LPS挑战的ALI模型中的作用。使用体外和体内实验进行了机理研究。用Tilianin（10和20μM）处理RAW264.7巨噬细胞和C57BL / 6小鼠，然后用LPS（1μg/ mL）诱导过夜。在用虫宁腹膜内治疗（10 mg / kg）之前，对C57BL / 6小鼠进行气管内LPS（2 mg / kg）给药以诱导ALI。体外，结果表明，Tilianin减少了LPS诱导的促炎细胞因子的分泌。在体内，用tilianin进行的预防性治疗减弱了巨噬细胞的浸润和组织病理学变化，并改善了炎症，如支气管肺泡灌洗液（BALF）中炎症介质的明显减少所显示。此外，tilianin有效抑制了MDA水平，ALI中GSH和SOD升高。总体而言，这些发现表明，tilianin是改善LPS诱导的ALI的潜在药物。