After an outbreak of classical scrapie in a dairy goat herd with over 1,800 goats, all goats in the herd were culled in 2008, cleaning and disinfection of the premises was implemented, and restocking with goats took place ~4 months after depopulation. Ten years later the new herd population is over 3,000 goats. This study was carried out to determine whether the measures were effective to prevent re-occurrence of scrapie to the 1% prevalence level seen when scrapie was first detected on this farm. A total of 280 goats with a minimum age of 18 months, which were predominantly at the end of their productive life, were euthanized, and brain and retropharyngeal lymph node examined by immunohistochemistry for disease-associated prion protein. Genotyping was done in all euthanized goats and live male goats used or intended for breeding to determine prion protein gene polymorphisms associated with resistance to classical scrapie. None of the goats presented with disease-associated prion protein in the examined tissues, and 34 (12.2%) carried the K₂₂₂ allele associated with resistance. This allele was also found in four breeding male goats. The study results suggested that classical scrapie was not re-introduced on this goat farm through mass restocking or inadequate cleaning and disinfection procedures. Further scrapie surveillance of goats on this farm is desirable to confirm absence of disease. Breeding with male goats carrying the K₂₂₂ allele should be encouraged to increase the scrapie-resistant population.

奶牛群中有1800多只山羊爆发了经典的刮ie病之后，该群中的所有山羊都在2008年被淘汰，对场所进行了清洁和消毒，在种群减少后约4个月进行了放牧。十年后，新的牧群数量超过了3,000只。进行这项研究是为了确定该措施是否有效地防止了瘙痒病的再次发生，使该农场首次发现瘙痒病时的流行率为1％。对总共280只最低年龄为18个月的山羊进行了安乐死，这些山羊主要在生产寿命结束时进行了安乐死，并通过免疫组织化学检查了大脑和咽后淋巴结中与疾病相关的pr病毒蛋白。在所有用于或打算进行育种的安乐死山羊和活雄山羊中进行基因分型，以确定与经典耐痒病有关的病毒蛋白基因多态性。在所检查的组织中，没有山羊表现出与疾病相关的病毒蛋白，并且有34只（12.2％）携带钾与抗性相关的_222个等位基因。该等位基因还在四只育种的公山羊中被发现。研究结果表明，该山羊场没有通过大量放养或没有足够的清洁和消毒程序来重新引入经典的刮板。需要进一步对该农场的山羊进行瘙痒病监测，以确认没有疾病。应鼓励携带K ₂₂₂等位基因的公山羊进行育种，以增加耐瘙痒病的种群。