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Physiological and molecular basis of promoting leaf growth in strawberry (Fragaria ananassa Duch.) by CO2 enrichment
Biotechnology & Biotechnological Equipment ( IF 1.4 ) Pub Date : 2020-01-01 , DOI: 10.1080/13102818.2020.1811766
Xuan Li 1 , Jing Zhao 1 , Mengya Shang 1 , Hongxia Song 1 , Jing Zhang 1 , Xiaoyong Xu 1 , Shaowen Zheng 1 , Leiping Hou 1 , Meilan Li 1 , Guoming Xing 1
Affiliation  

Abstract Strawberry (Fragaria ananassa Duch.) is an economically important crop. Its yield is highly dependent on photosynthesis, and carbon dioxide (CO2) is one of the most important constituents in photosynthesis. In this study, we investigated the physiological and molecular mechanisms of CO2 enrichment in increasing strawberry leaf growth, and ultimately fruit yield. Strawberry plants were grown under ambient CO2 (350–500 μmol/mol) and elevated CO2 (750–850 μmol/mol) in a controlled environment. The results showed that CO2 enrichment significantly increased the size, net photosynthetic rate and light saturation point of the leaves, but decreased the light compensation point of leaves. Using RNA sequencing (RNA-Seq), transcriptome profiling of leaf tissues under two CO2 concentrations was conducted. The analysis identified 150 differentially expressed genes in response to increased CO2 concentration; 14 of them were shown to be involved in photosynthesis. Gene Ontology (GO) enrichment and pathways analyses revealed that CO2 enrichment enhanced the biosysthesis of metabolism-promoting growth hormones; sped up the reduction step of CO2 assimilation, and increased the transport of photosynthetic products, which help explain the increased net photosynthetic rate and accelerated leaf growth under elevated CO2 level. These results provide a valuable reference for exploring the mechanism of CO2 application in strawberry production in greenhouses especially during offseason.

中文翻译:

富集 CO2 促进草莓 (Fragaria ananassa Duch.) 叶片生长的生理和分子基础

摘要 草莓(Fragaria ananassa Duch.)是一种重要的经济作物。其产量高度依赖于光合作用,而二氧化碳 (CO2) 是光合作用中最重要的成分之一。在这项研究中,我们研究了 CO2 富集促进草莓叶片生长和最终果实产量的生理和分子机制。草莓植物在受控环境中在环境 CO2 (350–500 μmol/mol) 和升高的 CO2 (750–850 μmol/mol) 下生长。结果表明,CO2富集显着增加了叶片的大小、净光合速率和光饱和点,但降低了叶片的光补偿点。使用 RNA 测序 (RNA-Seq),对两种 CO2 浓度下的叶组织进行转录组分析。分析确定了 150 个差异表达的基因,以响应二氧化碳浓度的增加;其中 14 种被证明参与了光合作用。基因本体论 (GO) 富集和通路分析表明,CO2 富集增强了促进新陈代谢的生长激素的生物合成;加快了 CO2 同化的还原步骤,并增加了光合产物的运输,这有助于解释在升高的 CO2 水平下净光合速率增加和叶片生长加速。这些结果为探索温室特别是淡季草莓生产中CO2施用机制提供了有价值的参考。基因本体论 (GO) 富集和通路分析表明,CO2 富集增强了促进代谢的生长激素的生物合成;加快了 CO2 同化的还原步骤,并增加了光合产物的运输,这有助于解释在升高的 CO2 水平下净光合速率增加和叶片生长加速。这些结果为探索温室特别是淡季草莓生产中CO2的应用机制提供了有价值的参考。基因本体论 (GO) 富集和通路分析表明,CO2 富集增强了促进代谢的生长激素的生物合成;加快了 CO2 同化的还原步骤,并增加了光合产物的运输,这有助于解释在升高的 CO2 水平下净光合速率增加和叶片生长加速。这些结果为探索温室特别是淡季草莓生产中CO2的应用机制提供了有价值的参考。
更新日期:2020-01-01
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