Epithelial membrane antigen (EMA) is one of the most widely used diagnostic immunohistochemical markers for human meningioma. To date, no published study on EMA expression in formalin‐fixed paraffin‐embedded (FFPE) tissue samples of canine meningioma is available. Here, we describe the results of an immunohistochemical study on 25 FFPE canine meningiomas using a monoclonal anti‐human EMA antibody. All meningiomas showed positive staining for EMA with cytoplasmic pattern, in nine cases associated with membranous staining. Area and intensity of staining were highly variable among cases. No clear relationships between tumour subtype/grade and area/intensity of staining were found. However, epithelial‐like patterns showed a higher affinity for EMA compared to the mesenchymal one. The present study provides the basis to explore the potential diagnostic application of this marker in canine meningioma. To investigate EMA expression in other central nervous system tumours of dogs are necessary to assess the specificity of this marker.

中文翻译：

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犬脑膜瘤中上皮膜抗原的免疫表达：新结果供前瞻性考虑

上皮膜抗原 (EMA) 是最广泛使用的人类脑膜瘤诊断免疫组织化学标志物之一。迄今为止，尚无关于犬脑膜瘤福尔马林固定石蜡包埋 (FFPE) 组织样本中 EMA 表达的已发表研究。在这里，我们描述了使用单克隆抗人 EMA 抗体对 25 个 FFPE 犬脑膜瘤进行的免疫组织化学研究的结果。所有脑膜瘤均显示 EMA 阳性染色并具有细胞质模式，其中 9 例与膜染色相关。不同病例的染色面积和强度差异很大。未发现肿瘤亚型/等级与染色面积/强度之间的明确关系。然而，与间充质的相比，上皮样模式对 EMA 显示出更高的亲和力。本研究为探索该标志物在犬脑膜瘤中的潜在诊断应用提供了基础。为了评估该标志物的特异性，有必要研究狗的其他中枢神经系统肿瘤中的 EMA 表达。