A pilot-scale upgraded gravity-driven submerged membrane (GDSM) reactor was constructed to enhance nitrogen removal. It was artificially formed multiple stratified environments (dissolved oxygen (DO) and substrate supply (TOC, TN, COD, NH₄⁺-N, NO₂⁻-N, and NO₃⁻-N)) by embedding moving water baffles to control water-flow process in bulk liquid with slow-flowing liquid state. Significant diversity and relative abundance of microorganisms associated with nitrogen transformation paths (i.e., ammonia-oxidizing archaea, ammonia-oxidizing bacteria, nitrite oxidizing bacteria, and denitrifying bacteria) were tailored to distribute on different spatial and temporal regions, and performed their dominant functions. The process simultaneously integrated diverse and effective nitrogen transformation paths (i.e., nitrification, partial nitrification, denitrification, anammox, and dissimilatory nitrate reduction) to achieve high nitrogen removal, with NH₄⁺-N, TN, and COD eliminated by 94.68 ± 2.55%, 55.16 ± 5.53%, and 80.17 ± 6.75%, respectively. Gene expressions involved in the nitrogen transformations were estimated by qPCR to explore the shifts of dominant nitrogen transforming bioreactions in multiple stratified environments. Pearson correlation coefficients supported that the functional genes had more stable and active ability by complementing each other. As a result, an endogenous integration of diverse nitrogen transformation paths was achieved in a single system by artificially tailoring the distributions of microbial communities and gene expressions with enhanced nitrogen removal.

建造了中试规模的重力驱动浸没式膜（GDSM）反应器，以提高脱氮率。它被人为地形成的多个分层环境（溶解氧（DO）以及基板供给（TOC，TN，COD，NH ₄ ⁺ -N，NO ₂ ^- -N和NO ₃ ^--N）），通过将移动的挡水板嵌入以控制具有缓慢流动状态的散装液体中的水流过程。定制与氮转化路径相关的微生物（即，氨氧化古细菌，氨氧化细菌，亚硝酸盐氧化细菌和反硝化细菌）的重要多样性和相对丰度，使其分布在不同的时空区域，并发挥其主导功能。该过程同时集成了多种有效的氮转化途径（例如硝化，部分硝化，反硝化，厌氧氨氧化和异化硝酸盐还原），以实现高脱氮率，并使用NH ₄ ⁺-N，TN和COD分别消除了94.68±2.55％，55.16±5.53％和80.17±6.75％。通过qPCR估计参与氮转化的基因表达，以探索在多个分层环境中显性氮转化生物反应的转变。皮尔逊相关系数支持功能基因彼此互补，具有更稳定和活跃的能力。结果，通过人工调整微生物群落的分布和增强氮去除的基因表达，在单个系统中实现了多种氮转化路径的内源整合。