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Evaluating the EPPO method for seed longevity analyses in Arabidopsis
Plant Science ( IF 5.2 ) Pub Date : 2020-12-01 , DOI: 10.1016/j.plantsci.2020.110644
Gonda Buijs , Leo A.J. Willems , Jan Kodde , Steven P.C. Groot , Leónie Bentsink

Seed longevity (storability) is an important seed quality trait. High seed quality is important in agriculture, for the industry, and for safeguarding biodiversity as many species are stored as seeds in genebanks. To ensure ex-situ seed survival, seeds are mostly stored at low relative humidity and low temperature. Oxidation is the main cause of seed deterioration in these dry storage conditions. The molecular mechanisms underlying dry seed survival remain poorly understood. Research on seed longevity is hampered by the lack of an experimental ageing method that mimics dry ageing well. Here, we propose the Elevated Partial Pressure of Oxygen (EPPO) method as the best available method to mimic and accelerate dry seed ageing. We have tested seed germination in Arabidopsis thaliana after EPPO storage at two different relative humidity (RH) conditions and confirm the large effect of oxygen and the seed moisture content on ageing during dry storage. Comparative Quantitative trait locus (QTL) analysis shows that EPPO at 55 % RH mimics dry ageing better than the commonly used Artificial Ageing and Controlled Deterioration tests at higher moisture levels.

中文翻译:

评估用于拟南芥种子寿命分析的 EPPO 方法

种子寿命(耐贮性)是一个重要的种子品质性状。高种子质量对农业、工业和保护生物多样性很重要,因为许多物种作为种子储存在基因库中。为确保异地种子成活,种子大多在低相对湿度和低温下储存。在这些干燥储存条件下,氧化是种子变质的主要原因。干种子存活的分子机制仍然知之甚少。由于缺乏模拟干老化的实验老化方法,对种子寿命的研究受到阻碍。在这里,我们建议将氧分压升高 (EPPO) 方法作为模拟和加速干种子老化的最佳可用方法。我们在两种不同的相对湿度 (RH) 条件下测试了 EPPO 储存后拟南芥种子的萌发情况,并证实了氧气和种子水分含量对干燥储存期间老化的巨大影响。比较定量性状位点 (QTL) 分析表明,在 55% RH 下的 EPPO 比在较高水分含量下常用的人工老化和受控老化测试更能模拟干老化。
更新日期:2020-12-01
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