Microorganisms are generally involved in the nucleation, growth and phase transformation of carbonate minerals, and influence the development of their morphology and polymorphism. However, understanding of the process of microbially induced carbonate precipitation (MICP) remains limited. Herein, MICP experiments were carried out using Curvibacter sp. HJ-1 and Arthrobacter sp. MF-2 in M2 medium, and the processes of MICP were monitored. Bacterial cells induced the precipitation of carbonate by creating favorable physicochemical conditions and acting as nucleation templates for carbonate particles and thereby, markedly influenced the morphology and growth of the carbonate structure. The extracellular polymeric substance (EPS) secreted by the bacteria was readily absorbed by the precipitated carbonate, which modified its crystal growth orientation. The MgCO₃ content of Mg-calcite, induced by MF-2, was dramatically higher than that induced by HJ-1; HJ-1 promoted the formation and stability of aragonite. Multiple formation mechanisms coexisted during the evolution process of the mineral morphologies in the presence of the bacteria. The spherulites observed mainly evolved from dumbbell-like precursors in the presence of MF-2, whereas aggregate growth was the main formation mechanism of radial spherulites in the presence of HJ-1.

微生物一般参与碳酸盐矿物的成核、生长和相变，并影响其形态和多态性的发展。然而，对微生物诱导碳酸盐沉淀（MICP）过程的了解仍然有限。在此，MICP 实验是使用Curvibacter sp.进行的。HJ-1和节杆菌sp. M2培养基中的MF-2，并监测MICP的过程。细菌细胞通过创造有利的物理化学条件并充当碳酸盐颗粒的成核模板来诱导碳酸盐的沉淀，从而显着影响碳酸盐结构的形态和生长。细菌分泌的胞外聚合物 (EPS) 很容易被沉淀的碳酸盐吸收，从而改变了其晶体生长方向。MgCO ₃MF-2诱导的Mg-方解石含量显着高于HJ-1诱导的Mg-方解石含量；HJ-1促进了文石的形成和稳定。在细菌存在下矿物形态演化过程中，多种形成机制并存。在MF-2存在下观察到的球晶主要由哑铃状前体演化而来，而在HJ-1存在下，聚集体生长是径向球晶的主要形成机制。