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A rapid GC method coupled with quadrupole or time of flight mass spectrometry for metabolomics analysis.
Journal of Chromatography B ( IF 3 ) Pub Date : 2020-09-02 , DOI: 10.1016/j.jchromb.2020.122355
Yanfeng Wang 1 , Lina Zhou 2 , Yang Zhou 3 , Chunxia Zhao 2 , Xin Lu 2 , Guowang Xu 2
Affiliation  

Gas chromatography-mass spectrometry (GC–MS) is an ideal tool for analyzing the intermediates of tricarboxylic acid cycle and glycolysis, sugars, organic acids and amino acids, etc. High-throughput metabolomics methods are required by large-scale clinical researches, and time of flight mass spectrometry (TOF MS) having fast scanning rate is preferable for rapid GC. Quadrupole MS (qMS) instruments have 95% market share, and their potential in rapid metabolomics is worth being studied. In this work, a within 15-min GC program was established and matched by qMS scanning for plasma metabolome analysis after N-methyl-N-(trimethylsilyl)-trifluoroacetamide derivatization. Compared to the longer-time program GC-qMS method, the rapid GC-qMS method had nearly no metabolome information loss, and it had excellent profile performance in repeatability, intra-day and inter-day precision, sampling range, linearity and extraction recovery. Compared to TOF MS, qMS achieved similar results in investigating lung cancer serum metabolic disruptions. Partial least squares-discriminant analysis revealed that the two datasets acquired by qMS and TOF MS had very similar model parameters, and most of top ranked differential metabolites were the same. This study provides a rapid and economical GC-qMS metabolomics method for researchers. Still, MS having faster scanning rate and higher sensitivity are recommended, if possible, to detect more small peaks and some co-eluted peaks.



中文翻译:

快速GC方法结合四极杆或飞行时间质谱进行代谢组学分析。

气相色谱-质谱(GC-MS)是分析三羧酸循环和糖酵解的中间体,糖,有机酸和氨基酸等的理想工具。大规模临床研究需要高通量代谢组学方法,并且具有快速扫描速率的飞行时间质谱(TOF MS)对于快速GC是优选的。四极质谱(qMS)仪器拥有95%的市场份额,其在快速代谢组学中的潜力值得研究。在这项工作中,建立了一个15分钟内的GC程序,并在N-甲基-N-(三甲基甲硅烷基)-三氟乙酰胺衍生化后通过qMS扫描进行血浆代谢组分析进行匹配。与较长时间的程序GC-qMS方法相比,快速GC-qMS方法几乎没有代谢组信息丢失,并且在重复性方面具有出色的分析性能,日间和日间精度,采样范围,线性度和提取回收率。与TOF MS相比,qMS在研究肺癌血清代谢破坏方面取得了相似的结果。偏最小二乘判别分析显示,qMS和TOF MS采集的两个数据集具有非常相似的模型参数,并且大多数排名最高的差异代谢物相同。这项研究为研究人员提供了一种快速,经济的GC-qMS代谢组学方法。不过,如果可能,仍建议使用具有更快扫描速度和更高灵敏度的质谱仪,以检测更多的小峰和一些共洗脱的峰。偏最小二乘判别分析显示,qMS和TOF MS采集的两个数据集具有非常相似的模型参数,并且排名靠前的大多数代谢物也相同。这项研究为研究人员提供了一种快速,经济的GC-qMS代谢组学方法。不过,如果可能,仍建议使用具有更快扫描速度和更高灵敏度的质谱仪,以检测更多的小峰和一些共洗脱的峰。偏最小二乘判别分析显示,qMS和TOF MS采集的两个数据集具有非常相似的模型参数,并且大多数排名靠前的差异代谢物相同。这项研究为研究人员提供了一种快速,经济的GC-qMS代谢组学方法。不过,如果可能,仍建议使用具有更快扫描速度和更高灵敏度的质谱仪,以检测更多的小峰和一些共洗脱的峰。

更新日期:2020-09-10
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