Abstract The aim of this study was to visualize non-enzymatic oxidative degradation damages in the initial decay stage of the brown rot fungus Rhodonia placenta degradation in furfuryl alcohol (FA) modified wood cell walls and untreated wood cell walls of radiata pine (Pinus radiata) sapwood. A decay test with small wood blocks (1.5 × 1.5 × 5 mm³) of untreated and furfurylated radiata pine selected from two different furfurylation processes was performed until the first mass loss occurred. The samples were exposed to the brown rot fungus R. placenta, monitored by light microscopy and analyzed topochemically by cellular UV microspectrophotometry (UMSP). The results showed that the FA modification process directly influenced: i) the fungal colonization and hyphal growth, ii) the spectral UV behavior, and iii) degradation patterns of the entire cell wall layers. For the first time, UMSP area scans and selective line scans of individual cell wall regions provide topochemical insights into oxidative degradation at the initial decay stage of furfuryl alcohol-modified P. radiata visualizing oxidative degradation in situ. Knowledge of the initial decay stage of brown rot degradation in FA-modified wood compared to untreated wood extends our understanding of the brown rot decay processes of cell wall compounds.

中文翻译：

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褐腐菌Rhodonia胎盘降解糠醇改性辐射松（Pinus radiata）初始衰变阶段非酶促氧化变化的拓扑化学和光学显微镜研究

摘要 本研究的目的是在糠醇 (FA) 改性的木材细胞壁和辐射松 (Pinus radiata) 的未处理木材细胞壁中观察褐腐病菌 Rhodonia 胎盘降解的初始腐烂阶段的非酶氧化降解损伤。边材。使用从两种不同糠化工艺中选出的未经处理和糠化辐射松的小木块 (1.5 × 1.5 × 5 mm³) 进行腐烂测试，直到发生第一次质量损失。将样品暴露于褐腐真菌 R. placenta，通过光学显微镜监测并通过细胞紫外显微分光光度法 (UMSP) 进行拓扑化学分析。结果表明，FA 改性过程直接影响：i) 真菌定植和菌丝生长，ii) 光谱 UV 行为，iii) 整个细胞壁层的退化模式。UMSP 区域扫描和单个细胞壁区域的选择性线扫描首次提供了对糠醇改性辐射假单胞菌初始衰变阶段氧化降解的拓扑化学见解，可视化原位氧化降解。与未经处理的木材相比，FA 改性木材中褐腐病降解的初始腐烂阶段的知识扩展了我们对细胞壁化合物褐腐腐烂过程的理解。