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In vitro assessment of some probiotic properties and inhibitory activity against Yersinia ruckeri of bacteria isolated from rainbow trout Oncorhynchus mykiss (Walbaum)
Aquaculture Reports ( IF 3.7 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.aqrep.2020.100447
Marco Medina , Giovanna Sotil , Violeta Flores , Carla Fernández , Nieves Sandoval

In the present study, we isolated bacteria from intestines of healthy rainbow trout, screened for their probiotic properties, and inhibitory activity against Y. ruckeri. A total of 21 out of 541 isolates from the intestine of adult rainbow trout (∼ 200–350 g), showed inhibition zones around at least one Y. ruckeri strains tested. Six of them were selected due to their ability to inhibit all the pathogenic strains on solid medium. They were identified as B. amyloliquefaciens 131and Paenibacillus spp (code 134, 1cc, 1d, 1k, and 2cc). Excluding the isolate 1k, selected strains were able to inhibit Y. ruckeri strains at 22 and 15 ± 0.5 °C when they were simultaneously cocultured, however, they did not show inhibition at 12 ± 0.5 °C. A pre incubation process of the selected strains for 24 h prior to co-culture with strains of Y. ruckeri enabled them to inhibit target pathogens at the lowest temperature tested. Furthermore, probiotic candidates survived to high concentrations of rainbow trout bile salts and acidic conditions. Selected bacteria were also able to produce amylolitic, proteolytic and lipolytic enzymes and showed variable hydrophobic values. Biosafety assays, performed by oral and intraperitoneal infection using B. amyloliquefaciens 131 and Paenibacillus spp (code 134, 1cc, 1d, 1k, and 2cc), showed no pathogenic effect in rainbow trout during 20-day experiment. Therefore, selected strains showed potential characteristics that are required to be evaluated in vivo for their possible subsequent use as probiotics in rainbow trout aquaculture.



中文翻译:

体外一些益生特性,并抑制活性的评估鲁氏耶尔森氏从虹鳟鱼分离的细菌的虹鳟(大麻哈鱼)

在本研究中,我们从健康的虹鳟鱼肠中分离出细菌,筛选了它们的益生菌特性和对R. ruckeri的抑制活性。在来自成年虹鳟鱼肠的541株分离菌中,总共21株(约200–350 g)在至少一种所测试的R. ruckeri菌株周围显示出抑制区。选择它们中的六个是因为它们具有抑制固体培养基上所有病原菌株的能力。它们被鉴定为解淀粉芽孢杆菌131和Paenibacillus spp(代码134、1cc ,1d,1k和2cc)。除分离株1k外,选定的菌株能够抑制R. ruckeri同时在22和15±0.5°C下进行共培养时,它们在12±0.5°C下没有显示抑制作用。在与R. ruckeri菌株共培养之前,将选定菌株进行24 h的预孵育过程使它们能够在最低测试温度下抑制靶标病原体。此外,益生菌候选物可以在高浓度的虹鳟鱼胆汁盐和酸性条件下存活。选定的细菌还能够产生淀粉酶,蛋白水解酶和脂解酶,并显示出可变的疏水值。通过使用解淀粉芽孢杆菌131和Paenibacillus进行的口腔和腹膜内感染进行的生物安全性测定spp(代码134、1cc,1d,1k和2cc)在20天的实验中未显示虹鳟的致病作用。因此,选定的菌株表现出潜在的特性,需要对其进行体内评估,用于随后可能作为虹鳟鱼水产养殖中的益生菌使用。

更新日期:2020-09-01
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