Acta Tropica ( IF 2.7 ) Pub Date : 2020-09-01 , DOI: 10.1016/j.actatropica.2020.105683 J M Lee 1 , S H Yek 2 , R F Wilson 2 , S Rahman 1
Understanding the diversity and dynamics of the microbiota within the mosquito holobiome is of great importance to apprehend how the microbiota modulates various complex processes and interactions. This study examined the bacterial composition of Aedes albopictus across land use type and mosquito sex in the state of Selangor, Malaysia using 16S rRNA sequencing. The bacterial community structure in mosquitoes was found to be influenced by land use type and mosquito sex, with the environment and mosquito diet respectively identified to be the most likely sources of microbes. We found that approximately 70% of the microbiota samples were dominated by Wolbachia and removing Wolbachia from analyses revealed the relatively even composition of the remaining bacterial microbiota. Furthermore, microbial interaction network analysis highlighted the prevalence of co-exclusionary patterns in all networks regardless of land use and mosquito sex, with Wolbachia exhibiting co-exclusionary interactions with other residential bacteria such as Xanthomonas, Xenophilus and Zymobacter.
中文翻译:
白纹伊蚊(双翅目:葫芦科)全缘组的特征:马来西亚土地利用类型和蚊虫性的细菌组成。
了解灭蚊全菌群中微生物群的多样性和动力学对于理解微生物群如何调节各种复杂的过程和相互作用非常重要。这项研究使用16S rRNA测序技术检查了马来西亚雪兰莪州不同土地利用类型和蚊子性的白纹伊蚊的细菌组成。发现蚊子中细菌的群落结构受土地利用类型和蚊子性别的影响,其中环境和蚊食分别被确定为最可能的微生物来源。我们发现大约70%的微生物菌群样品由Wolbachia占据,并去除Wolbachia分析表明,其余细菌菌群的组成相对均匀。此外,微生物相互作用网络分析突出显示了所有网络中共排斥模式的普遍性,无论土地利用和蚊虫性别如何,沃尔巴克菌与其他居住细菌如Xanthomonas,Xenophilus和Zymobacter表现出共排斥相互作用。