Lactobacillus paracasei SD1, Lactobacillus rhamnosus SD11, and Lactobacillus gasseri SD12 were proposed as potential probiotics for oral health. However, the effects of them on host physiology are still unknown. This study aimed to select strains that can promote host growth and development in monocolonized Drosophila model compared with axenic and the commercial Lactobacillus rhamnosus GG–treated flies. The morphogenesis and growth of axenic Drosophila melanogaster were assessed from embryo to adult stage when provided with each probiotic strain–supplemented food. The colonization and persistence of probiotic in fly gut were also evaluated. The results indicated that axenic condition caused the lowest adult weight and emergence rate. All probiotic groups had higher weight than axenic group. Lact. rhamnosus SD11 group presented high adult emergence rate equivalent to Lact. rhamnosus GG group, which is significantly higher than the others. However, Lact. gasseri SD12 group had significantly lower adult weight and emergence rate when compared with Lact. rhamnosus GG group. The gut probiotics levels were rapidly increased within the first day after receiving probiotics. After probiotic cessation, their number in gut decreased and was maintained at low level, except for Lact. gasseri SD12, which completely vanished since day one. In conclusion, Lact. paracasei SD1, Lact. rhamnosus SD11, and Lact. rhamnosus GG can affect morphogenesis and weight of flies when fed since immature stage and have short period gut colonization. The findings of this study could possibly imply comparable health-promoting effects between Lact. paracasei SD1 and Lact. rhamnosus SD11 to commercial strain in Drosophila model.

副干酪乳杆菌SD1、鼠李糖乳杆菌SD11 和加氏乳杆菌SD12 被提议作为口腔健康的潜在益生菌。然而，它们对宿主生理的影响仍然未知。本研究旨在与无菌和商业鼠李糖乳杆菌GG 处理的果蝇相比，选择能够促进单克隆果蝇模型中宿主生长和发育的菌株。无菌黑腹果蝇的形态发生和生长当提供每种益生菌菌株补充食品时，对从胚胎到成年阶段进行评估。还评估了益生菌在苍蝇肠道中的定殖和持久性。结果表明，无菌条件导致成虫体重和羽化率最低。所有益生菌组的体重均高于无菌组。乳酸。鼠李糖SD11组呈现出相当于Lact的高成虫率。鼠李糖GG组明显高于其他组。然而，拉克。与Lact相比，gasseri SD12 组的成年体重和羽化率显着降低。鼠李糖GG组。肠道益生菌水平在接受益生菌后的第一天迅速增加。停用益生菌后，它们在肠道中的数量减少并保持在低水平，乳酸除外。gasseri SD12，从第一天起就完全消失了。总之，Lact。副干酪SD1，乳酸。鼠李糖SD11 和乳酸。鼠李糖GG 可以影响果蝇的形态发生和体重，从未成熟阶段开始喂养，并具有短期肠道定植。这项研究的结果可能意味着Lact之间具有可比性的健康促进作用。副干酪SD1 和Lact。鼠李糖SD11 到果蝇模型中的商业菌株。