Abstract

Methods of diode laser spectroscopy were applied for monitoring of fast physiological variations in the content of endogenous carbon monoxide (CO) in exhaled air. To this end, a CO analyzer based on a tunable diode laser emitting radiation around 2.35 μm at room temperature was constructed. CO concentration in exhaled air was determined by measuring resonance absorption of CO molecule at the R(6) line of the 0–2 band in the vicinity of 4285 cm^–1. Using this line that is well separated from the nearby absorption lines of Н₂О enables high selectivity of CO analysis under the conditions of high humidity of analyzed air. The possibility of monitoring fast physiological variations of CO content in exhaled air of humans and animals by means of the developed system was demonstrated in studies involving a number of physiological load tests, such as breathing pure oxygen (both humans and rats), physical load, and hyperventilation. The dynamics of CO release caused by certain pharmaceutical drugs was also studied. It is demonstrated that laser diagnostics of CO in the spectral region near 2.35 μm allows detecting fast dynamic processes of release of this gas molecule that represents one of the signaling and metabolite molecules in exhaled air when conducting various physiological and medical studies, investigating trends and interrelation of processes responsible for production, transport, buffering, and release of CO in an organism.

中文翻译：

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用二极管激光光谱法监测呼出空气中内源性CO含量的快速变化

摘要

二极管激光光谱法被用于监测呼出空气中内源性一氧化碳（CO）含量的快速生理变化。为此，构建了基于可调谐二极管激光器的CO分析仪，该二极管分析仪在室温下发射约2.35μm的辐射。呼出空气中的CO浓度是通过测量4285 cm ^–1附近0-2谱带的R（6）线处的CO分子的共振吸收来确定的。使用此线是从良好的Н附近吸收线分离₂О可在高分析空气湿度条件下实现高选择性的CO分析。通过涉及许多生理负荷测试的研究，证明了通过发达的系统监测人和动物呼出空气中CO含量快速生理变化的可能性，例如呼吸纯氧（人和大鼠），身体负荷，和过度换气。还研究了某些药物引起的CO释放动力学。事实证明，在进行各种生理和医学研究时，对2.35μm附近光谱区域的CO进行激光诊断可以检测出呼出气体中代表信号和代谢物分子之一的这种气体分子的释放的快速动态过程，