Serum samples of healthy rats and rats with inoculated tumors on days 14 and 28 after transplantation of cholangiocarcinoma cells were analyzed using Raman spectroscopy (RS). It was shown that the intensity of the amide 1 band differs in the serum spectra depending on the stage of tumor development and correlates with the protein concentration in the samples. In some animals, there is a significant increase in the blood glucose concentration, leading to glycation of proteins and a change in the shape of the amide 1 band. The appearance of a band at 1670 cm^–1 is most pronounced in the spectra of samples on the 28th day of the experiment and may be associated with an increase in the content of β-structural elements in the protein conformation. Using the principal component analysis, we assessed the general differences in the Raman spectra of the blood serum in rats of control groups and rats on the 28th day after tumor transplantation.

中文翻译：

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拉曼光谱法研究移植性胆管癌大鼠的血清

使用拉曼光谱法（RS）分析健康大鼠以及在胆管癌细胞移植后第14和28天有肿瘤的大鼠的血清样品。结果表明，酰胺1带的强度在血清谱中随肿瘤发展阶段的不同而不同，并且与样品中的蛋白质浓度相关。在某些动物中，血糖浓度显着增加，导致蛋白质糖基化和酰胺1带形状的改变。乐队在1670厘米^{–1处的出现}在实验的第28天，样品的光谱中最明显的“β-结构”可能与蛋白质构象中β结构元素的含量增加有关。使用主成分分析，我们评估了对照组和肿瘤移植后第28天大鼠的血清拉曼光谱的一般差异。