Highly purified chitosan was generally preferred for heavy metal (HM) removal and the preparation parameters varied largely without any agreement. This study investigated to the influences of chitin with different purities on the HM removal of corresponding chitosan. Sea shrimp waste was used as raw materials and Pb²⁺ was used as target HM. The results of orthogonal experimental analysis showed that only acid concentration played an important role in the deproteinization and demineralization processes of the chitin preparation under HCl, H₂SO₄ and CH₃COOH treatment. Ca-bearing minerals (CBM) but not free –NH₂ group of chitosan played a major role in the removal of Pb²⁺ from solution. Partly purified chitosan mainly removed Pb²⁺ by precipitation and then biosorption. The dissociation of Ca²⁺ from CBM elevated pH value of Pb²⁺ solution which benefited to precipitation and the formation of NH₂-Pb²⁺. Partly purified chitosan prepared from HCl and CH₃COOH treated chitin showed 720–753 mg/g of Pb²⁺ adsorption at the initial pH value of 6.0; however, highly purified chitosan prepared from HCl treated chitin showed only 45–160 mg/g. Chitosan prepared from H₂SO₄ treated chitin showed 720–752 mg/g of Pb²⁺ adsorption. This research found the unexplored information for the industrial application of chitosan with minimum cost but the highest HM removal efficiency.

高度纯化的壳聚糖通常是去除重金属 (HM) 的首选，并且制备参数变化很大，没有任何一致意见。本研究考察了不同纯度的几丁质对相应壳聚糖脱除HM的影响。以海虾废料为原料，以Pb ²⁺为目标重金属。正交实验分析结果表明，仅酸浓度在HCl、H ₂ SO ₄和CH ₃ COOH处理下的几丁质制剂的脱蛋白和脱矿过程中起重要作用。含钙矿物 (CBM) 但不是游离的壳聚糖–NH ₂基团在去除 Pb ^{2+ 中}起主要作用从解决方案。部分纯化的壳聚糖主要通过沉淀和生物吸附去除 Pb ²⁺。Ca ²⁺从煤层气中解离提高了Pb ²⁺溶液的pH值，有利于沉淀和NH ₂ -Pb ²⁺的形成。由 HCl 和 CH ₃ COOH 处理的几丁质制备的部分纯化壳聚糖在初始 pH 值为 6.0 时表现出 720–753 mg/g Pb ²⁺吸附；然而，由 HCl 处理的几丁质制备的高度纯化的壳聚糖仅显示 45-160 mg/g。由 H ₂ SO ₄处理的几丁质制备的壳聚糖显示 720–752 mg/g Pb ²⁺吸附。本研究为壳聚糖的工业应用发现了成本最低但HM去除效率最高的未开发信息。