An electrochemical biosensor for organophosphorus pesticides detection has been developed by immobilizing acetylcholinesterase (AChE) enzyme, through a multi-step functionalization process, on porous silicon (PSi) surface. The PSi surface was functionalized using thermal hydrosilylation creating acidic chains to stabilize the surface. The AChE was covalently attached on the PSi surface and exhibited a good enzyme activity in the presence of its substrate acetylthiocholine iodide (ATI) by forming thiocholine. The response of the device shows a linear evolution over a wide concentration range of ATI from 0.15 to 0.65 mM with a good correlation coefficient. Furthermore, AChE revealed high affinity to its substrate ATI from calculated Michaelis–Menten constant (K_m), which is about 0.249 mM. The effect of the organophosphorus pesticide malathion was monitored by measuring the current oxidation in ATI solution. After incubation, it shows a drastic decrease in the electrochemical response in the presence of the pesticide at different concentrations (2–8 nM).

通过将乙酰胆碱酯酶（AChE）酶通过多步功能化过程固定在多孔硅（PSi）表面上，已经开发出了一种用于有机磷农药检测的电化学生物传感器。使用热氢化硅烷化功能对PSi表面进行功能化，生成酸性链以稳定表面。AChE共价附着在PSi表面上，并在其底物乙酰硫代胆碱碘化物（ATI）存在下通过形成硫代胆碱表现出良好的酶活性。该器件的响应显示出在从0.15到0.65 mM的宽范围ATI浓度范围内具有良好相关系数的线性演变。此外，AChE从计算的Michaelis–Menten常数（K _m），约为0.249 mM。通过测量ATI溶液中的电流氧化来监测有机磷农药马拉硫磷的作用。孵育后，在不同浓度（2–8 nM）的农药存在下，它显示出电化学反应的急剧下降。