Indian Journal of Microbiology ( IF 3 ) Pub Date : 2020-09-02 , DOI: 10.1007/s12088-020-00906-2 Arely Nohemí López-Anchondo 1, 2, 3 , Daniel López-de la Cruz 1, 2, 3 , Edgar Gutiérrez-Reyes 1 , José Cristobal Castañeda-Ramírez 4 , Norma Margarita De la Fuente-Salcido 1, 2, 3
Fungi are the primary infectious agents in plant crops and many post-harvest fungal diseases of fruit and vegetables causing significant economic losses worldwide. Here, the antifungal effect of Prosopis glandulosa extract (PgE) against phytopathogenic fungi was evaluated. The effect with PgE (5, 4, 2, 1, 0%) as AI (%) and radial growth rate reduction (Kr %) were determined in vitro in Colletotrichum gloeosporoides, Fusarium oxysporum, Rhizopus oryzae and R. stolonifer (1 × 105 spores/mL). The phytopathogenicity of fungal strains was performed under in vivo conditions (room temperature, 25–30 °C and refrigeration, ~ 4 °C) by fruit surface inoculation method on strawberries, tomatoes and carrots by recording the development of mycelial growth, necrosis, soft rot and dehydration symptoms showed on each fruit at 14 days. The extract (5%) showed the highest AI against C. gloesporioides (~ 96%), and F. oxysporum (~ 79%) and growth rate reduction of 74.92% and 64.82% respectively. Likewise, the extract controls the development of phytopathogenicity symptoms against C. gloesporioides and F. oxysporum in vivo conditions, nevertheless, was less efficiency against both Rhizopus species. The P. glandulosa extract represents an efficient, economical, and eco-friendly alternative to preserve the quality of the agricultural products and to increase their shelf life.
中文翻译:
牧豆树提取物(Prosopis glandulosa)对植物病原真菌的体外和体内抗真菌活性
真菌是植物作物和许多水果和蔬菜收获后真菌病害的主要传染原,在全球范围内造成重大经济损失。在这里,评估了牧羊草提取物 ( Pg E) 对植物病原真菌的抗真菌作用。以Pg E (5, 4, 2, 1, 0%) 作为 AI (%) 和径向生长速率降低 (K r %) 的影响在体外测定了炭疽病菌、尖孢镰刀菌、米根霉和R. stolonifer ( 1×10 5孢子/mL)。真菌菌株的植物致病性是在体内条件下(室温,25-30°C 和冷藏,~4°C)通过果实表面接种法在草莓、西红柿和胡萝卜上通过记录菌丝体生长、坏死、软在 14 天时,每个水果都会出现腐烂和脱水症状。提取物 (5%) 对C. gloesporioides (~ 96%) 和F. oxysporum (~ 79% ) 表现出最高的 AI ,并且生长速率分别降低了 74.92% 和 64.82%。同样,该提取物在体内条件下控制了针对C. gloesporioides和F. oxysporum的植物病原性症状的发展,然而,针对根霉属的效率较低物种。P. glandulosa提取物代表了一种有效、经济和环保的替代品,可以保持农产品的质量并延长其保质期。