The research and the individuation of tumour markers in biological fluids are currently one of the main tools to support diagnosis, prognosis, and monitoring of the therapeutic response in oncology. Although the identification of tumour markers in asymptomatic patients is crucial for early diagnosis, its application is still limited by the relatively low sensitivity and the complexity of existing methods (i.e. ELISA, mass spectrometry). We developed an easy, fast, and ultrasensitive surface-enhanced Raman scattering (SERS)–based system, for the detection and quantitation of the LGALS3BP (90K) biomarker that was used as a model, based on the development of antibody-functionalized nanostructured gold surfaces. The detection system was effective for the ultrasensitive detection and characterization of samples of different biochemical compositions. In conclusion, this work could provide the foundation for the development of a medical diagnostic device with the highest predictive power when compared with the methods currently used in cancer diagnostics.

生物流体中肿瘤标志物的研究和个体化目前是支持肿瘤学中治疗反应的诊断，预后和监测的主要工具之一。尽管在无症状患者中鉴定肿瘤标志物对于早期诊断至关重要，但其应用仍然受到相对较低的灵敏度和现有方法（即ELISA，质谱）的复杂性的限制。基于抗体功能化纳米结构金的开发，我们开发了一种简单，快速和超灵敏的基于表面增强拉曼散射（SERS）的系统，用于检测和定量用作模型的LGALS3BP（90K）生物标志物表面。该检测系统对于不同生化成分的样品的超灵敏检测和表征是有效的。