Direct contact-based coculture of human dermal fibroblasts and epidermal keratinocytes has been a long-standing and challenging issue owing to different serum and growth factor requirements of the two cell types. Existing protocols employ high serum concentrations (up to 10% fetal bovine serum), complex feeder systems and a range of supplemental factors. These approaches are technically demanding and labor intensive, and pose scientific and ethical limitations associated with the high concentrations of animal serum. On the other hand, serum-free conditions often fail to support the proliferation of one or both cell types when they are cultured together. We have developed two reduced serum approaches (1-2% serum) that support the contact-based coculture of human dermal fibroblasts and immortalized keratinocytes and enable the study of cell migration and wound closure.

中文翻译：

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基于接触的人真皮成纤维细胞和表皮角质形成细胞共培养的减少血清方法。

由于两种细胞类型对血清和生长因子的需求不同，人真皮成纤维细胞和表皮角质形成细胞的直接接触共培养一直是一个长期存在且具有挑战性的问题。现有方案采用高血清浓度（高达 10% 胎牛血清）、复杂的饲养系统和一系列补充因素。这些方法技术要求高、劳动强度大，并造成与高浓度动物血清相关的科学和伦理限制。另一方面，当它们一起培养时，无血清条件通常不能支持一种或两种细胞类型的增殖。