A novel fiber optofluidic laser biosensor based on nanoparticle-functionalized thin-walled hollow optical fiber is developed for sensitive detection of enzyme horseradish peroxidase (HRP). High sensitivity is achieved thanks to the high surface-to-volume ratio of the nanomaterials. Two kinds of attractive nanomaterials, i.e., Au nanorods and SiO2 nanoparticles, are selected as demonstrative models to be coated onto the inner surface of thin-walled hollow fiber through electrostatic adsorption and biochemical cross-linking, respectively, while the streptavidin-biotin conjugation mechanism acts as a bridge for the specific recognition of enzyme. The nanoparticle-functionalized fiber optofluidic lasers have lower lasing threshold, compared to the control group without nanomaterial. We experimentally demonstrate that the nanomaterials enhanced fiber optofluidic laser biosensors can detect HRP in the range of 75–15 000 pM with a good linearity in semi-logarithmic scale, and with a sensitivity enhancement of 57.4% by using the SiO2 nanoparticles. We believe that this nanoparticle-enhanced fiber optofluidic laser biosensor could provide an efficient strategy for biomolecule detection such as enzyme-linked immunosorbent assay (ELISA).

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用于灵敏酶检测的纳米材料增强型光纤光流体激光生物传感器

开发了一种基于纳米颗粒功能化薄壁中空光纤的新型光纤光流体激光生物传感器，用于灵敏检测辣根过氧化物酶 (HRP)。由于纳米材料的高表面积与体积比，实现了高灵敏度。选择了两种有吸引力的纳米材料，即 Au 纳米棒和 SiO2 纳米粒子作为示范模型，分别通过静电吸附和生化交联将其包覆到薄壁中空纤维的内表面，而链霉亲和素-生物素共轭机制充当特定识别酶的桥梁。与没有纳米材料的对照组相比，纳米颗粒功能化的光纤光流体激光器具有较低的激光阈值。我们通过实验证明纳米材料增强的光纤光流体激光生物传感器可以检测 75-15 000 pM 范围内的 HRP，在半对数尺度上具有良好的线性，并且通过使用 SiO2 纳米粒子，灵敏度提高了 57.4%。我们相信这种纳米粒子增强型光纤光流体激光生物传感器可以为生物分子检测提供有效的策略，例如酶联免疫吸附测定 (ELISA)。