Background: Indocyanine green (ICG) has received considerable interest as a biocompatible organic photothermal agent, and curcumin (Cur) is considered an attractive natural chemopreventive and chemotherapeutic compound. However, the in vivo applicability of ICG and Cur is significantly restricted by their poor ability to target tumors and their extremely low solubility.
Materials and Methods: To address these problems, ICG/Cur-loaded albumin nanoparticles (ICG-BSA-Cur-NPs) based on the nab^TM (nanoparticle albumin-bound) technology were applied to neuroblastomas in vivo.
Results: The fabricated ICG-BSA-Cur-NPs were found to be spherical, ∼ 150 nm in size and highly dispersible and stable in aqueous solution. Approximately 80% of the incorporated ICG and Cur were gradually released from the NPs over 48 h. All formulations of ICG-BSA-Cur-NPs (5∼ 20 μg/mL) showed efficient hyperthermia profiles (up to 50– 60°C within 5 min) in response to 808-nm NIR laser irradiation in vitro and in vivo. Notably, ICG-BSA-Cur-NPs illuminated with 808-nm laser irradiation (1.5 W/cm²) showed excellent cytotoxicity toward N2a cells in vitro and undisputable antitumor efficacy in N2a-xenografted mice in vivo, compared to other tested sample groups (tumor volumes for PBS, BSA-Cur-NPs, free ICG, and ICG-BSA-Cur-NPs groups were 1408.6 ± 551.9, 1190.6 ± 343.6, 888.6 ± 566.2, and 103.0 ± 111.3 mm³, respectively).
Conclusion: We demonstrate that these hyperthermal chemotherapeutic ICG-BSA-Cur-NPs have potential as a future brain tumor treatment.



中文翻译：

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基于近红外诱导热疗的吲哚菁绿和姜黄素共载纳米火球状白蛋白纳米颗粒用于肿瘤消融。

背景：吲哚菁绿（ICG）作为一种生物相容性有机光热剂引起了相当大的兴趣，姜黄素（Cur）被认为是一种有吸引力的天然化学预防和化学治疗化合物。然而，ICG 和 Cur 的体内适用性受到其靶向肿瘤能力差和溶解度极低的显着限制。
材料和方法：为了解决这些问题，将基于 nab ^TM（纳米白蛋白结合）技术的载有 ICG/Cur 的白蛋白纳米颗粒 (ICG-BSA-Cur-NPs) 应用于体内神经母细胞瘤。
结果：发现制造的 ICG-BSA-Cur-NPs 是球形的，尺寸约为 150 nm，在水溶液中高度分散和稳定。大约 80% 的 ICG 和 Cur 在 48 小时内逐渐从 NP 中释放出来。ICG-BSA-Cur-NPs (5∼20 μg/mL) 的所有制剂在体外和体内对 808-nm NIR 激光照射的响应均显示出有效的热疗曲线（5 分钟内达到 50-60°C）。值得注意的是，与其他测试样品组相比，用 808 nm 激光照射 (1.5 W/cm ² ) 照射的 ICG-BSA-Cur-NPs 在体外对 N2a 细胞表现出优异的细胞毒性，在体内对 N2a 异种移植小鼠具有无可争议的抗肿瘤功效。 PBS、BSA-Cur-NPs、游离 ICG 和 ICG-BSA-Cur-NPs 组的肿瘤体积分别为 1408.6 ± 551.9、1190.6 ± 343.6、888.6 ± 566.2 和 103.0 ± 111.3 mm ³， 分别）。
结论：我们证明这些超热化疗 ICG-BSA-Cur-NPs 具有作为未来脑肿瘤治疗的潜力。