Trillium tschonoskii is a medicinal plant known to biosynthesize steroidal saponins. A phytochemical investigation of the rhizomes of T. tschonoskii led to the isolation of nine new furostanol saponins (1–9) and 11 known analogues (10–20). Five of these new compounds were shown to have hydroxy groups at the C-5 and C-6 positions, while two possess a rare aglycone containing carbonyl groups at the C-16 and C-22 positions as well as a Δ¹⁷⁽²⁰⁾ double bond, and the others have conjugated double bonds in the E-ring or have different sugar chains at the C-3 position. All the isolates were tested for their effect on the expansion of human cord blood (CB) CD34⁺ hematopoietic stem and progenitor cells. It was found that CB CD34⁺ cells treated with compounds 6, 7, 9, 10, 14, 15, and 19 showed increased numbers of rigorously phenotype-defined hematopoietic stem cells. Notably, compounds 9, 10, 13, and 14 demonstrated an enhanced ability to increase the percentages and numbers of CB CD34⁺CD38^– cells and multipotential progenitors. The present study is the first to report that furostanol saponins from T. tschonoskii rhizomes can promote hematopoietic stem/progenitor cell (HSPC) expansion.

中文翻译：

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延龄草中的呋喃甾醇皂苷促进人脐带血造血干细胞和祖细胞的扩增。

延龄草是一种已知可生物合成甾体皂苷的药用植物。的根茎的植物化学物质调查T. tschonoskii导致的九个新呋甾皂苷（隔离1 - 9和11个的已知类似物（）10 - 20）。这些新化合物中有五个被证明在 C-5 和 C-6 位置具有羟基，而两个在 C-16 和 C-22 位置具有含有羰基的罕见苷元以及 Δ ¹⁷⁽²⁰⁾双键，其他的在E环上有共轭双键或在C-3位有不同的糖链。测试了所有分离株对人脐带血 (CB) CD34 ⁺扩增的影响造血干细胞和祖细胞。据发现，CB CD34 ⁺用化合物处理细胞6，7，9，10，14，15，和19显示出增加的严格表型定义的造血干细胞的数目。值得注意的是，化合物9，10，13，和14显示增加CB CD34的百分比和数量的增强的能力⁺ CD38 ^-细胞和多能祖细胞。本研究首次报道了T. tschonoskii 的呋甾醇皂苷 根状茎可促进造血干/祖细胞 (HSPC) 扩增。