Background. Various chemical agents have been used as an adjuvant treatment for giant cell tumor (GCT). However, the comparative effect of these chemicals remains unclear. Methods. Multinucleated and spindle cells from cultured GCT patients, characterized by Nanog and Oct4 expression with RT-PCR, were directly administered, in vitro, with concentrations of 1%, 3%, and 5% of H₂O₂ and 75%, 85%, and 95% of ethanol for 10 minutes and concentrations of 0.003%, 0.005%, 0.01%, 0.03%, 0.1%, and 0.3% of H₂O₂ for 5 minutes and were incubated for 24 hours. Cell morphology, cell viability, and flow cytometry after various concentrations of H₂O₂ and ethanol exposure were assessed. Results. H₂O₂ in all concentrations caused loss of cell viability. The number of viable cells after H₂O₂ exposure was related to the concentration-dependent effect. The initial viable spindle-shaped cell, multinucleated giant cell, and round-epithelioid cell had morphological changes into fragmented nonviable cells after exposure to H₂O₂. Flow cytometry using Annexin V showed cell death due to necrosis, with the highest concentration amounting to 0.3%. Conclusion. Administering local chemical adjuvants of H₂O₂ in vitro caused loss of viable GCT cells. The number of viable cells after H₂O₂ exposure was related to the concentration-dependent effect, whereas reducing concentration of H₂O₂ may cause loss of viability and morphology of cultured GCT cells with the apoptotic mechanism.

中文翻译：

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化学试剂对巨细胞肿瘤的细胞毒性机制：一项体外研究。

背景。各种化学试剂已被用作巨细胞瘤 (GCT) 的辅助治疗。然而，这些化学物质的比较效果仍不清楚。方法。来自培养的 GCT 患者的多核细胞和梭形细胞，其特征是通过 RT-PCR 表达 Nanog 和 Oct4，在体外直接施用浓度为 1%、3% 和 5% 的 H ₂ O ₂和 75%、85% , 和 95% 的乙醇 10 分钟和 0.003%、0.005%、0.01%、0.03%、0.1% 和 0.3% 的 H ₂ O ₂浓度5 分钟并孵育 24 小时。_{不同浓度 H 2} O ₂后的细胞形态、细胞活力和流式细胞术和乙醇暴露进行了评估。结果。所有浓度的H ₂ O ₂都会导致细胞活力丧失。_{H 2} O ₂暴露后的活细胞数量与浓度依赖性效应有关。最初的有活力的梭形细胞、多核巨细胞和圆形上皮样细胞在暴露于H ₂ O ₂后形态发生变化，变成破碎的无活力细胞。使用 Annexin V 的流式细胞术显示细胞因坏死而死亡，最高浓度为 0.3%。结论。_{施用 H 2} O ₂的局部化学助剂在体外导致活的 GCT 细胞丢失。_{H 2} O ₂暴露后的活细胞数量与浓度依赖性效应有关，而降低H ₂ O ₂浓度可能导致具有凋亡机制的培养的GCT细胞的活力和形态丧失。