We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ∼150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin-sensitive and -resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

中文翻译：

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代谢途径和信号传导的简单靶向分析：靶向蛋白质组学的强大工具。

我们介绍了STAMPS，这是一种以通路为中心的网络服务，用于开发目标蛋白质组学检测方法。STAMPS通过提供几个直观的界面来指导用户，以进行快速，简化的方法设计。将我们精心设计的框架应用于信号传导和代谢途径，我们将平均分析开发时间减少了约150倍，并揭示了胰岛素信号传导受胰岛素敏感和抗药性状态下蛋白质丰度变化的有效控制。尽管在当前状态下，STAMPS主要包含鼠标数据，但其设计目的是易于扩展其他生物。