A novel photodeactivation strategy for controlling gene expression has been developed based on light-induced activation of cAMP response element binding protein (CREB). Light-induced cleavage of the photoresponsive protecting group of an antagonist of CREB binding protein (CBP) results in photocleaved products with weak binding affinity for CBP. This photodissociation reaction enables protein–protein interactions between CBP and CREB that trigger the formation of a multiprotein transcription complex to turn gene expression “on”. This enables irradiation of antagonist-treated HEK293T cells to be used to trigger temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression under photolytic control.

中文翻译：

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用于控制CREB依赖性基因表达的可光灭活拮抗剂。

基于光诱导的cAMP响应元件结合蛋白（CREB）的激活，已经开发了一种控制基因表达的新型光灭活策略。CREB结合蛋白（CBP）拮抗剂的光响应保护基的光诱导裂解导致对CBP的结合亲和力弱的光裂解产物。这种光解离反应使CBP和CREB之间的蛋白质相互作用可以触发多蛋白质转录复合物的形成，从而使基因表达“打开”。这使得能够使用拮抗剂处理的HEK293T细胞进行辐照，以触发在光解控制下CREB依赖性转录活性和内源基因表达的暂时恢复。