Mesenchymal stem cells (MSCs) are being used to treat many diseases as they exhibit great regenerative potential. However, MSC’s transplantation sometimes does not yield the maximum regenerative outcome as they are unable to survive in inflammatory conditions. Several approaches including preconditioning are used to improve the survival rate of mesenchymal stem cells. One such recently reported approach is preconditioning MSCs with plant extracts. The present study was designed to evaluate the effect of Daphne mucronata extract on stressed human adipose-derived mesenchymal stem cells (hADMSCs). Isolated hADMSCs were preconditioned with different concentrations of Daphne muconata extract and the protective, proliferative, antioxidant and anti-inflammatory effect was assessed through various assays and expression analysis of inflammatory markers regulated through NF-κB pathway. Results suggest that preconditioning hADMSCs with Daphne mucronata increased the cell viability, proliferative and protective potential of hADMSCs with a concomitant reduction in LDH, ROS and elevation in SOD activity. Moreover, both the ELISA and gene expression analysis demonstrated down regulations of inflammatory markers (IL1-β, TNF-α, p65, p50, MMP13) in Daphne mucronata preconditioned hADMSCs as compared to stress. This is the first study to report the use of MIA induced oxidative stress against hADMSC’s and effect of Daphne mucronata on stressed hADMSCs. Results of these studies provided evidence that Daphne mucronata protects the hADMSCs during stress conditions by down regulating the inflammatory markers and hence increase the viability and proliferative potential of hADMSCs that is crucial for transplantation purposes.

间充质干细胞（MSCs）具有巨大的再生潜力，正被用于治疗许多疾病。但是，MSC的移植有时无法获得最大的再生结果，因为它们无法在炎症条件下存活。包括预处理在内的几种方法可用于提高间充质干细胞的存活率。最近报道的一种方法是用植物提取物预处理MSC。本研究旨在评估水蚤（Daphne mucronata）提取物对应激的人脂肪间充质干细胞（hADMSCs）的影响。分离的hADMSC用不同浓度的达芙妮粘膜预处理通过各种测定和通过NF-κB途径调节的炎症标志物的表达分析，对提取物的保护，增殖，抗氧化和抗炎作用进行了评估。结果表明，用Daphne mucronata预处理hADMSCs可以提高hADMSCs的细胞活力，增殖和保护潜力，同时降低LDH，ROS和SOD活性。此外，ELISA和基因表达分析均显示，与应激相比，达芙妮（daphne mucronata）预处理的hADMSCs中炎症标志物（IL1-β，TNF-α，p65，p50，MMP13）的下调。这是第一个报道MIA诱导的针对hADMSC的氧化应激和Daphne mucronata的作用的研究在紧张的hADMSC上。这些研究结果提供了证据，达芙妮在下调条件下通过调节炎症标志物来保护hADMSCs，从而增加了hADMSCs的活力和增殖潜力，这对于移植至关重要。